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Invitrogen
Cathepsin L Monoclonal Antibody (33/1), eBioscience™
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FIGURE: 1 / 6
Cathepsin L Antibody (BMS166) in WB
Knockout of Cathepsin L was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1042354_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Cathepsin L was performed by loading 30 µg of A549 Cas9 (Lane 1), andA549 Cathepsin L KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More
Product Details
BMS166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
33/1
Immunogen
Rec. Human Cathepsin
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: Cathepsin L is a lysosomal cysteine proteinase consisting of a heavy chain of about 25 kDa and a light chain of about 5 kDa derived proteolytically from the same precursor. Cathepsin L has been shown to be produced by many different cell types. Since cathepsin L is capable of degrading protein constituents of the extracellular matrix it is assumed to play a crucial role in tumor progression and metastasis and a number of other disorders where the destruction of the extracellular matrix is the major cause of disease (e.g. rheumatoid arthritis, neurodegeneration).
Applications Tested: ELISA, Western Blotting.
Purity: >95%.
Target Information
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alternatively spliced; Cathepsin L1; CTSL; hCATL AIII; lysosomal cysteine protease; Major excreted protein; MEP; Procathepsin L; unnamed protein product
Gene Aliases: CATL; CTSL; CTSL1; MEP
UniProt ID: (Human) P07711
Entrez Gene ID: (Human) 1514
adaptive immune response
proteolysis
protein autoprocessing
fusion of virus membrane with host plasma membrane
receptor-mediated endocytosis of virus by host cell
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
collagen catabolic process
zymogen activation
enkephalin processing
fusion of virus membrane with host endosome membrane
CD4-positive, alpha-beta T cell lineage commitment
viral entry into host cell
antigen processing and presentation of peptide antigen
proteolysis involved in cellular protein catabolic process
elastin catabolic process
macrophage apoptotic process
cellular response to thyroid hormone stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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