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Invitrogen
BPI Polyclonal Antibody
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FIGURE: 1 / 1
BPI Antibody (PA5-92405) in WB
Western blot analysis of BPI in extracts of various cell lines. Samples were incubated with BPI Polyclonal antibody (Product # PA5-92405) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-92405
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 248-487 of human BPI (NP_0017162)
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: AETLDVQMKG EFYSENHHNP PPFAPPVMEF PAAHDRMVYL GLSDYFFNTA GLVYQEAGVL KMTLRDDMIP KESKFRLTTK FFGTFLPEVA KKFPNMKIQI HVSASTPPHL SVQPTGLTFY PAVDVQAFAV LPNSSLASLF LIGMHTTGSM EVSAESNRLV GELKLDRLLL ELKHSNIGPF PVELLQDIMN YIVPILVLPR VNEKLQKGFP LPTPARVQLY NVVLQPHQNF LLFGADVVYK; Positive Samples: Jurkat, HepG2, SK-Hep1, Mouse liver, Mouse thymus; Cellular Location: Cytoplasmic granule membrane
Target Information
The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. The binding of BPI to lipid A causes multiple anti-infective activities against gram-negative bacteria. These include: cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, neutralization of gram-negative bacterial LPS, opsonization of bacteria to enhance phagocytosis by neutrophils. BPI may be a critical determinant in the development of LPStriggered airways disease since airway epithelial cells constitutively express the BPI gene and produce the BPI protein. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21 kDa bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bactericidal permeability-increasing protein; bactericidal/permeability-increasing protein; BPI; BPI fold containing family D, member 1; CAP 57; recombinant BPI holoprotein, rBPI; RP13-218F6_A.1
Gene Aliases: 9230105K17Rik; BPI; BPIFD1; rBPI
UniProt ID: (Human) P17213, (Mouse) Q67E05
Entrez Gene ID: (Human) 671, (Mouse) 329547
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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