Invitrogen

BRD1 Polyclonal Antibody

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BRD1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of MCF7 cells using Product # PA5-19829, anti-BRD1 antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).

BRD1 Antibody in Western Blot (WB) — Western blot analysis of TE 671 Whole Cell Lysate using Product # PA5-19829, BRD1 primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Raji Whole Cell Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Product Details

PA5-19829

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1 µg/mL	-
Immunocytochemistry (ICC/IF)	1 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human BRD1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10974150

Product Specific Information

For Western Blot, this antibody has non-specific bands at 34 kDa and 92 kDa.

This antibody is predicted to react with chicken, Xenopus laevis and zebrafish based on sequence homology.

Target Information

This gene encodes a protein of unknown function. The protein contains a bromodomain, a sequence motif often found in transcriptional coactivators, and localizes to the nucleus in testis and several other cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BR140-like protein; Bromodomain and PHD finger-containing protein 2; Bromodomain-containing protein 1; DKFZp686F0325; RP3-522J7.4

Gene Aliases: 1110059H06Rik; AI316859; BRD1; BRL; BRPF1; BRPF2; Kiaa4191; mKIAA4191

UniProt ID: (Human) O95696, (Mouse) G5E8P1

Entrez Gene ID: (Human) 23774, (Mouse) 223770

Molecular Function: chromatin/chromatin-binding, or -regulatory protein DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator