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Invitrogen
BRD1 Polyclonal Antibody
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FIGURE: 1 / 1
BRD1 Antibody (PA5-97274) in WB
Western blot analysis of BRD1 in extracts of various cell lines. Samples were incubated with BRD1 Polyclonal antibody (Product # PA5-97274) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-97274
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human BRD1 (NP_0553921)
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Amount
54 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MRRKGRCHRG SAARHPSSPC SVKHSPTRET LTYAQAQRMV EIEIEGRLHR ISIFDPLEII LEDDLTAQEM SECNSNKENS ERPPVCLRTK RHKNNRVKKK NEALPSAHGT PASASALPEP KVRIVEYSPP SAPRRPPVYY KFIEKSAEEL DNEVEYDMDE EDYAWLEIVN EKRKGDCVPA VSQSMFEFLM DRFEKESHCE NQKQGEQQSL IDEDAVCCIC MDGECQNSNV ILFCDMCNLA VHQECYGVPY; Positive Samples: HeLa, Jurkat; Cellular Location: Nucleus
Target Information
This gene encodes a protein of unknown function. The protein contains a bromodomain, a sequence motif often found in transcriptional coactivators, and localizes to the nucleus in testis and several other cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AF10 family member; BR140-like protein; BRD1; Bromodomain and PHD finger-containing protein 2; Bromodomain-containing protein 1; DKFZp686F0325; RP3-522J7.4; unnamed protein product
Gene Aliases: BRD1; BRL; BRPF1; BRPF2
UniProt ID: (Human) O95696
Entrez Gene ID: (Human) 23774
protein binding
zinc ion binding
histone acetyltransferase activity (H3-K14 specific)
histone acetyltransferase activity (H4-K5 specific)
histone acetyltransferase activity (H4-K8 specific)
histone acetyltransferase activity (H4-K12 specific)
metal ion binding
unmodified histone reader activity
histone reader activity
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to immobilization stress
erythrocyte maturation
positive regulation of erythrocyte differentiation
regulation of developmental process
response to electrical stimulus
regulation of hemopoiesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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