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Invitrogen
BRD4 Polyclonal Antibody
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FIGURE: 1 / 7
BRD4 Antibody (PA5-100998) in IHC (P)
Immunohistochemistry analysis of BRD4 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with BRD4 polyclonal antibody (Product # PA5-100998) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-100998
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BRD4(Accession O60885), corresponding to amino acid residues T28-Q78.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815847
Product Specific Information
Antibody detects endogenous levels of total Brd4.
Target Information
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BRD4-NUT FUSION; bromodomain-containing 4; bromodomain-containing 5; Bromodomain-containing protein 4; chromosome-associated protein; fsh-like protein; MCAP; Mitotic chromosome-associated protein; Protein HUNK1
Gene Aliases: BRD4; Brd5; CAP; HUNK1; HUNKI; MCAP; WI-11513
UniProt ID: (Human) O60885, (Mouse) Q9ESU6
Entrez Gene ID: (Human) 23476, (Rat) 362844, (Mouse) 57261
regulation of transcription involved in G1/S transition of mitotic cell cycle
transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of G2/M transition of mitotic cell cycle
viral process
covalent chromatin modification
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
regulation of phosphorylation of RNA polymerase II C-terminal domain
inner cell mass cell proliferation
chromatin remodeling
protein phosphorylation
chromosome segregation
positive regulation of DNA binding
histone H4-K12 acetylation
histone H3-K14 acetylation
negative regulation of DNA damage checkpoint
regulation of transcription, DNA-templated
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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