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Product Details
OSB00075W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide to from aa region 710-750 of mouse BRSK1 conjugated to an immunogenic carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: BRSK1.
Target Information
BRSK1 (SAD1) is a serine/threonine kinase, and is a UV-induced DNA damage checkpoint kinase. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BR serine/threonine-protein kinase 1; Brain-selective kinase 1; Brain-specific serine/threonine-protein kinase 1; protein kinase SAD1A; SAD1 homolog; SAD1 kinase; SAD1A; SadB kinase short isoform; serine/threonine kinase SAD-B; serine/threonine kinase SADB; Serine/threonine-protein kinase BRSK1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1
Gene Aliases: BRSK1; Gm1100; hSAD1; KIAA1811; RGD1563268; SAD-B; SAD1; SADB
UniProt ID: (Human) Q8TDC3, (Rat) B2DD29, (Mouse) Q5RJI5
Entrez Gene ID: (Human) 84446, (Rat) 499073, (Mouse) 381979
magnesium ion binding
protein serine/threonine kinase activity
ATP binding
protein kinase binding
gamma-tubulin binding
tau-protein kinase activity
protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
protein phosphorylation
cellular response to DNA damage stimulus
neurotransmitter secretion
axonogenesis
response to UV
response to ionizing radiation
establishment of cell polarity
neuron differentiation
G2 DNA damage checkpoint
intracellular signal transduction
centrosome duplication
protein autophosphorylation
neuron projection morphogenesis
G2/M transition of mitotic cell cycle
cell cycle
nervous system development
phosphorylation
peptidyl-serine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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