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Product Details
PA5-103338
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:3,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A synthesized peptide derived from human BRSK2(Accession Q8IWQ3), corresponding to amino acid residues S245-I271. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2852683 |
Target Information
BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C, and CDC25B in an in vitro assay, but a kinase-dead mutant does not. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BR serine/threonine-protein kinase 2; brain serine/threonine kinase 2; Brain-selective kinase 2; Brain-specific serine/threonine-protein kinase 2; protein kinase SAD1B; SAD1B; Serine/threonine-protein kinase 29; Serine/threonine-protein kinase BRSK2; Serine/threonine-protein kinase SAD-A; testicular tissue protein Li 30
Gene Aliases: 4833424K13Rik; BRSK2; C11orf7; HUSSY-12; Kiaa4256; PEN11B; SAD-A; SAD1; SADA; STK29
UniProt ID: (Human) Q8IWQ3, (Mouse) Q69Z98
Entrez Gene ID: (Human) 9024, (Mouse) 75770, (Rat) 293631
Molecular Function:
non-receptor serine/threonine protein kinase
G2/M transition of mitotic cell cycle
protein phosphorylation
exocytosis
mitotic nuclear division
axonogenesis
peptidyl-serine phosphorylation
establishment of cell polarity
neuron differentiation
actin cytoskeleton reorganization
ERAD pathway
regulation of ATPase activity
cell division
regulation of insulin secretion involved in cellular response to glucose stimulus
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
regulation of retrograde protein transport, ER to cytosol
apoptotic process
cell cycle
nervous system development
phosphorylation
intracellular signal transduction
neuron projection morphogenesis
cellular response to DNA damage stimulus
response to UV
response to ionizing radiation
magnesium ion binding
protein serine/threonine kinase activity
ATP binding
protein kinase binding
tau-protein kinase activity
ATPase binding
ATPase regulator activity
nucleotide binding
protein kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
Performance Guarantee
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