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Beclin 1 Polyclonal Antibody

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Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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Beclin 1 Antibody in Immunocytochemistry (ICC/IF) — Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (bs-55024R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Beclin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:100 overnight at 4°C, DAB staining.

Beclin 1 Antibody in Western Blot (WB) — Lane 1:U-87MG cell lysates; Lane 2: Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Mouse Spleen lysates; Lane 5: Rat ovary lysates; Lane 6: Rat Lung cell lysates probed with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Product Details

BS-55024R

Applications	Tested Dilution	Publications
Western Blot (WB)	1:300-5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-400	-
Immunocytochemistry (ICC/IF)	1:50-200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 1% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways - PI3KC3-C1 is involved in the initation of autophagosomes and PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis. Beclin 1 also plays a role in antiviral host defense.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APG6; ATG6 autophagy related 6 homolog; Bcl-2-interacting protein beclin; beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin 1 (coiled-coil, myosin-like BCL2-interacting protein); beclin 1, autophagy related; Beclin-1; Beclin-1-C 35 kDa; Beclin-1-C 37 kDa; Coiled-coil myosin-like BCL2-interacting protein; coiled-coil, myosin-like BCL2-interacting protein; Protein GT197; testis secretory sperm-binding protein Li 215e

Gene Aliases: 4921513J16Rik; 5430417M23Rik; ATG6; beclin1; BECN1; GT197; VPS30

UniProt ID: (Human) Q14457, (Rat) Q91XJ1, (Mouse) O88597

Entrez Gene ID: (Human) 8678, (Rat) 114558, (Mouse) 56208

Molecular Function: protease inhibitor