Invitrogen

Bif1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (9) Bif1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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Bif1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Bif1 in human testis tissue shows distinct cytoplasmic positivity in cells in seminiferus ducts. Samples were probed using a Bif1 Polyclonal Antibody (Product # PA5-54111).

Bif1 Antibody in Western Blot (WB) — Western blot analysis of Bif1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a Bif1 Polyclonal Antibody (Product # PA5-54111).

Bif1 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of Bif1 demonstrates antibody specificity. Immunohistochemical analysis of Bif1 using anti-Bif1 Polyclonal Antibody (Product # PA5-54111), shows significant staining of Bif1 in human testis and shows minimal or weak staining in human pancreas tissues. The relative expression levels of Bif1 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Bif1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2638554

Product Specific Information

Immunogen sequence: THAHHLRCLN DFVEAQMTYY AQCYQYMLDL QKQLGSFPSN YLSNNNQTSV TPVPSVLPNA IGSSAMASTS GLVITSPSNL SDLKECSGSR KARVLYDYDA ANSTELSLLA DEVITVFSVV GMDSD

Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 87%.

Target Information

Bif1 gene encodes a protein similar to a protein in rodents which is induced by bone morphogenic protein 2 in vitro and may be involved in BMP2-induced transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bax-interacting factor 1; Bif-1; CGI-61; dJ612B15.2; Endophilin-B1; PPP1R70; protein phosphatase 1; protein phosphatase 1, regulatory subunit 70; regulatory subunit 70; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2 like endophilin B1; SH3-domain GRB2-like B1 (endophilin); SH3-domain GRB2-like endophilin B1; sh3glb1 {ECO:0000312|EMBL:AAH79085.1}; testicular tissue protein Li 172

Gene Aliases: Bif-1; CGI-61; dJ612B15.2; KIAA0491; PPP1R70; SH3GLB1

UniProt ID: (Human) Q9Y371, (Rat) Q6AYE2

Entrez Gene ID: (Human) 51100, (Rat) 292156

Molecular Function: G-protein modulator enzyme modulator guanyl-nucleotide exchange factor