Invitrogen

Blimp-1 Polyclonal Antibody

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Blimp-1 Antibody in Western Blot (WB) — Western blot analysis of Blimp-1 by a Blimp-1 monoclonal antibody (Product # PA5-19216) at a concentration of 0.5 µg/mL. A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Blimp-1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Blimp-1 in A431 cells using a polyclonal antibody (Product #PA5-19216). A431 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

Blimp-1 Antibody in Immunocytochemistry (ICC/IF) — Figure 2 DENV suppressed NLRP12 expression by up-regulating PRDM-1 expression in a manner dependent on the TBK1/IRF3 and NF-kappaB signaling pathways. (A, B) hMDMs were infected with DENV (MOI=4) for indicated time periods and PRDM1 expression was determined by qPCR and western blot. The gray value of PRDM1 protein bands was analyzed by Image J gel image analysis software, and the relative grayscale value was normalized to that of beta-actin. (C) hMDMs were treated with Poly ( I:C ) and harvested for PRDM1 expression analysis. (D) The siRNA knockdown of PRDM1 was performed in hMDMs and the mRNA level of PRDM1 was measured. (E) PRDM1-silenced hMDMs were infected with DENV for 48h (MOI=4) and NLRP12 expression was determined via qPCR. (F) dTHP1 cells were infected with DENV with an MOI of 4 for 72h. Immunofluorescence staining was performed for DENV E protein, NLRP12, PRDM1 and DAPI (Bar: 10mum). (G) dTHP1 cells were transiently transfected with the siRNAs against the PRDM1, and then PRDM1-Flag-expressing vectors were transfected into the cells. After DENV infection at a MOI of 4 for 72h, the expression levels of NLRP12 and PRDM1 were detected by western blot analysis. The gray value of PRDM1 protein and NLRP12 bands was analyzed by Image J gel image analysis software and the relative grayscale value was normalized to that of beta-actin. (H, I) hMDMs cells were pre-treated with the inhibitors for TBK1 (BX795) or NF-kappaB (JSH-23) and subjected to DENV infection at a MOI of 4 f

Product Details

PA5-19216

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

3-5 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

10 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DISDNADRLEDVED) corresponding to the internal amino acids of PRDM1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10984847

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus Response.

Frontiers in immunology

Li X,Dong Z,Liu Y,Song W,Pu J,Jiang G,Wu Y,Liu L,Huang X

Published figure using Blimp-1 polyclonal antibody (Product # PA5-19216) in Immunocytochemistry

Mon Feb 07 00:00:00 EST 2022

Bioinformatics

Protein Aliases: B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; RP1-134E15.1

Gene Aliases: BLIMP1; PRDI-BF1; PRDM1

UniProt ID: (Human) O75626

Entrez Gene ID: (Human) 639

Molecular Function: C2H2 zinc finger transcription factor