Invitrogen

Blimp-1 Polyclonal Antibody

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Blimp-1 Antibody in Western Blot (WB) — Western blot analysis of Blimp-1 by a Blimp-1 monoclonal antibody (Product # PA5-19216) at a concentration of 0.5 µg/mL. A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Blimp-1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Blimp-1 in A431 cells using a polyclonal antibody (Product #PA5-19216). A431 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

Product Details

PA5-19216

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-1.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	3-5 µg/mL	-
Flow Cytometry (Flow)	10 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (DISDNADRLEDVED) corresponding to the internal amino acids of PRDM1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10984847

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; RP1-134E15.1

Gene Aliases: BLIMP1; PRDI-BF1; PRDM1

UniProt ID: (Human) O75626

Entrez Gene ID: (Human) 639

Molecular Function: C2H2 zinc finger transcription factor