Invitrogen

Blimp-1 Polyclonal Antibody

Product Image Gallery

Blimp-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Blimp-1 on paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Blimp-1 polyclonal antibody (Product# PA5-79861) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Blimp-1 Antibody in Western Blot (WB) — Western blot analysis of Blimp-1 in Lane 1: rat thymus tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: rat stomach tissue lysate, Lane 4: rat lung tissue lysate, Lane 5: mouse thymus tissue lysate, Lane 6: mouse spleen tissue lysate, Lane 7: mouse stomach tissue lysate, Lane 8: mouse lung tissue lysate, Lane 9: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with Blimp-1 polyclonal antibody (Product # PA5-79861) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human PRDM1/Blimp1 recombinant protein (Position: M1-Q230).

Conjugate

Unconjugated

Form

Lyophilized

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

RRID

AB_2746976

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP-1; BLIMP1; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1

Gene Aliases: b2b1765Clo; Blimp-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1

UniProt ID: (Human) O75626, (Mouse) Q60636

Entrez Gene ID: (Human) 639, (Mouse) 12142, (Rat) 309871

Molecular Function: DNA binding protein centromere DNA-binding protein nucleic acid binding