Invitrogen

Blimp-1 Monoclonal Antibody (6D3), eBioscience™

Product Image Gallery

Blimp-1 Antibody — Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82)

Blimp-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 µg/mL of Anti-Human/Mouse Blimp1 Purified or 10 µg/mL of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) followed by Anti-Rat IgG Biotin, and DAB visualization.

Blimp-1 Antibody in Western Blot (WB) — Immunoblotting of reduced cell lysates prepared from A20 cell line using 5 µg/mL of Anti-Human/Mouse Blimp1 Purified. Bands were visualized using Anti-Rat IgG HRP.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Western Blot (WB)

1-10 µg/mL

Published Applications

Flow Cytometry (Flow)

See 1 publication below

Western Blot (WB)

See 6 publications below

Immunofluorescence (IF)

See 4 publications below

ChIP assay (ChIP)

See 2 publications below

Immunohistochemistry (IHC)

See 5 publications below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host / Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6D3

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_1907437

Product Specific Information

Description: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection.

Applications Reported: This 6D3 antibody has been reported for use in immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections.

Applications Tested: This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blottiing, this antibody can be used at 1-10 µg/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B Lymphocyte Induced Maturation Protein 1; B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; BLIMP1; Positive Regulatory Domain I Binding Factor 1; Positive regulatory domain I-binding factor 1; PR Domain Containing 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; PRDM1

Gene Aliases: b2b1765Clo; Blimp-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1

UniProt ID: (Human) O75626, (Mouse) Q60636

Entrez Gene ID: (Human) 639, (Mouse) 12142

Molecular Function: DNA binding protein centromere DNA-binding protein nucleic acid binding