Invitrogen

Blimp-1 Monoclonal Antibody (6D3), eBioscience™

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Blimp-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 µg/mL of Anti-Human/Mouse Blimp1 Purified or 10 µg/mL of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) followed by Anti-Rat IgG Biotin, and DAB visualization.

Blimp-1 Antibody in Western Blot (WB) — Immunoblotting of reduced cell lysates prepared from A20 cell line using 5 µg/mL of Anti-Human/Mouse Blimp1 Purified. Bands were visualized using Anti-Rat IgG HRP.

Blimp-1 Antibody in Immunocytochemistry (ICC/IF) — Figure 2 Similar gene expression profile and germ cell-specific protein level of hPGCLCs generated via 0.35% MC method and U96 method. (A) Quantitative analysis of the expression of specific genes in EpCAM-/INTEGRINalpha6-high cells (day 4) via U96 method and 0.35% MC method, respectively. The error bars represent mean +- SD with three biological replicates (white, iMeLC; green, U96; red, MC). (B) Immunofluorescence analysis of OCT4, TFAP2C, BLIMP1, and SOX17 on day 4 embryoids from 0.35% MC method and U96 method. The nuclei were stained with Hoechst (left). Scale bars, 20 mum. (C) The proportion of Sox17 (+) cells at day 4 EBs via 0.35% MC method and U96 method (right); gray, U96; dark green, 0.35% MC; ** P < 0.01.

Blimp-1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Extended Data Fig.8 Effect of NANOG on hPGC induction, characterization of NANOS3-tdTomato reporter hPSC containing inducible SOX17, BLIMP1 with or without TFAP2C, and similarity between Cytokine- and SOX17/BLIMP1-induced hPGC a. Represents overexpression of Dex-inducible NANOG transgenes in NT reporter Comp-hPSC. b. Day4 embryoids following induction of NANOG (by Dex), with or without cytokines as indicated. c. FACS patterns after induction of hPGCs; NT/AP +ve cells (%) shown in Extended Data Fig. 8b. d. Represents overexpression of Dex-inducible SOX17, Dox-inducible BLIMP1, Shield1(S1)-inducible TFAP2C transgenes in NT reporter Comp-hPSC. e. Immunostaining of NT reporter Comp-hPSC +iSBT 1 day after induction of SOX17, BLIMP1 and TFAP2C by addition of Dex, Dox or S1. Scale bar: 50 um. f. Immunostaining of NT reporter Comp-hPSC +iSB 1 day after induction of SOX17 or BLIMP1 by addition of Dex or Dox. Scale bar: 50 um. g . Immunostaining of day 2 embyroid induced with or without Dex to induce nuclear localization of SOX17. Notably, accumulation of SOX17 signal is observed in +Dex condition. h. Changes in gene expression (RT-qPCR) during hPGC induction: Comp-hPSCs control (AP +ve cells); NT +ve hPGCs induced by SOX17/BLIMP1 or Cytokines; NT -ve cells in cells exposed to Cytokines. i. Unsupervised hierarchical clustering (UHC) of gene expression. j. Gene set enrichment analysis (GSEA) of 123 hPGC-specific genes ( Supplementary Table 1 ) on the transcriptome o

Blimp-1 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F)) — Figure 3 CD38 Expression in Human Germ-Cell-Related Cells and Epigenetic Changes in hPGCLCs (A) FACS analysis of NANOS3-mCherry and CD38 on WIS2-NANOS3-mCherry cell line cultured in 4i medium and on day 4 and 5 embryoids following hPGCLC induction. Ratios of CD38 low and high expression in the NANOS3-mCherry-positive cells are indicated. (B) FACS histogram of CD38 low and high populations in TCam-2. (C) FACS analysis of CD38 and TNAP on genital ridges isolated from a week 6 human embryo. (D) Expression analysis by RT-qPCR for FACS-sorted TNAP-positive 4i hESCs (TNAP+ hESC) and CD38 low or high/NANOS3-mCherry day 5 hPGCLCs. Relative expression levels are shown with normalization to beta-ACTIN . Error bars indicate mean +- SD from two independent biological replicates. (E and F) Immunofluorescence analysis for 5hmC (E) and TET1 (F) on day 4 embryoids cryosection. OCT4 or BLIMP1 were used to identify hPGCLCs (highlighted). Scale bars, 50 mum. (G) Quantification of immunofluorescence intensity of various epigenetic marks/modifiers in hPGCLCs and somatic neighbors in day 1-4 embryoids (see also Figures S3 A-S3C). For UHRF1, only KI-67-positive (proliferating) cells were used for quantification. Numbers below each box denotes number of cells analyzed. Black central line represents the median, boxes and whiskers represent the 25 th and 75 th , and 2.5 th and 97.5 th percentiles, respectively. Wilcoxon signed-rank test was used to test for statistical significance. #p < 0.05; * p < 0

Blimp-1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure S5 Generation of BLIMP1 KO in WIS2-NANOS3-mCherry hESC Line, Related to Figure 5 (A) Targeting strategy of BLIMP1 knockout in hESC with the designated guide RNA (gRNA) and the resulting deleted sequences. (B) Immunofluorescence of SOX17, NANOS3-mCherry and OCT4 (upper panel); and BLIMP1, NANOG and TFAP2C on wild-type (WT) and BLIMP1 KO day 4 embryoids. Scale bars = 70 mum.

Blimp-1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Fig. 4. Macrophage-derived MCPIP1 regulates B-lymphocyte expansion in lymphoid organs. (A-D) Spleen sections were stained with anti-mouse IRF4, BLIMP1, CD95 and B220 antibodies and quantified by Adobe Photoshop software as percentage of positively stained high-power field (HPF) from eight mice per group; *** P <0.001. (magnification x200, A,B; x400, C; x100, D) (E) The proliferative activity of primary B lymphocytes stimulated with serum from wild-type and knockout mice. Data are means+-s.e.m. (F) The proliferative activity of A20 B-lymphocyte cell line stimulated with serum from wild-type and knockout mice and CpG. Data are means+-s.e.m. ; *** P <0.001.

Blimp-1 Antibody in Immunohistochemistry (IHC) — Figure 2 Recapitulating dermal cell fate decision towards DC fate. (A) Diagram deciphering dermal lineage and epidermal lineage differentiation at E13.5 to E16.5. (B) Pseudotime ordering of all dermal cell lineage cells. Each dot represents one cell and each branch represents one cell state. The left plot was labeled with developmental time and the right plot was labeled with cell states. (C) Heatmap illustrating the (differentially expressed genes, DEGs) dynamics towards DC and fibroblast fate along pseudotime. The DEGs were clustered into 4 gene sets according to k -means and the expression curve was illustrated in the middle. GO terms enriched for each gene set were labeled in the right panel. DC fated represents cell fate 2 in Figure 2 B, while dermal adipocyte progenitors represent cell fate 1 in Figure 2 B. (D) Immunofluorescence analysis of PRDM1, SOX9, LEF1, PCNA, CTNNB1, KRT15 and BMP2 expression in the E16.5 dorsal skin. Scale bars, 50 mum. (E) SCENIC binary regulon activity heatmap depicting DC and fibroblast enriched regulons. The column depicts a single cell while the row depicts regulons. For the regulons of particular interest, their representative binding motif was visualized in the right panel. ""On"" depicts active, while ""Off"" represents inactive. (F) Sequential visualization of enriched regulon activity in each gene set corresponding to Figure 2 C. Their representative target genes were also provided and the red ticks depict confirmed markers in DC fate

Blimp-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig 2 The eGFP-tagged Blimp1-fusion protein is efficiently expressed in the developing placenta and embryonic small intestine, but fails to rescue germ cell defects. (A) Immunohistochemical staining of E9.5 placentae demonstrates the eGFP-tagged Blimp1-fusion protein is correctly expressed in the spongiotrophoblast layer. The position of the central maternal artery is outlined. Bar, 200 mum. SpT, spongiotrophoblast; dec, maternal decidua; lab, labyrinth trophoblast. (B) Western blot analysis demonstrates robust expression in E16.5 small intestine. RIPA lysates of STO fibroblasts are included as a negative control. Levels of Blimp1 expression were quantified relative to the first wild type littermate. (C) Immunohistochemical staining demonstrates nuclear expression of both endogenous and eGFP-tagged Blimp1 protein in E16.5 villus epithelium. Bar, 200 mum. (D) The eGFP-tagged Blimp1 knock-in allele fails to reconstitute germ cell formation. Fast red alkaline phosphatase staining at E7.5 demonstrates markedly reduced numbers of PGCs (black arrows). Hematoxylin- and eosin-stained sections confirm that adult homozygous BEG/BEG testes lack spermatocytes. Bar, 200 mum.

Blimp-1 Antibody in Flow Cytometry (Flow) — Extended Data Fig.9 Response of SOX17 KO Comp-hPSC to SOX17 a. Overexpression of Dex-inducible SOX17 (iS) in SOX17 KO Comp-hPSC. b. Gene expression (RT-qPCR) on day 4 of FACS-sorted NANOS3-mCherry (NC)/Alkaline phosphatase (AP) +ve hPGCs by RT-qPCR. c. FACS analysis of d2 embryoids induced from WT Comp-hPSC, SOX17 KO Comp-hPSC and SOX17KO Comp-hPSC rescued with SOX17GR transgene (iS) (% CXCR4/AP+ cells). d. FACS pattern of day 2 embyoid induced from NT reporter Comp-hPSC showing AP/CXCR4 +ve cells expressing NT. e. Represents SOX17 inducible system (iSdd). Expression of SOX17 fused with destabilized domain (DD) can be induced by Doxycycline (Dox); addition of Shield1 (S1) can stabilize SOX17-DD protein. f. Western blots showing SOX17 expression level in day 5 embryoids from SOX17KO+iSdd Comp-hPSC. Embryoids were induced with Cytokines. To induce SOX17, different concentration of Dox and S1 were added. As controls, NC/AP +ve hPGCs and NC/AP -ve cells from WT Comp-hPSC-derived embryoids induced with Cytokines were used. Histone H3 (H3) was used for internal control. g. Immunostaining of day 4 embryoids from SOX17KO+iSdd Comp-hPSC. Embryoids from SOX17 KO and WT Comp-hPSC induced with Cytokines were used as controls. Scale bar: 50 mum. h. Quantification of immunostaining data in Extended Data Fig. 9g. The numbers of OCT4/BLIMP1 +ve hPGCs, FOXA2 +ve endodermal cells and OCT4/BLIMP1 +ve hPGCs expressing FOXA2 were counted from 3 different embryoids. The proportions of the 3 popula

Blimp-1 Antibody in ChIP assay (ChIP) — Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in ChIP assay

Product Details

14-5963-82

Applications	Tested Dilution	Publications
Western Blot (WB)	1-10 µg/mL	View 8 publications 8 publications
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	10 µg/mL	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 4 publications 4 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ChIP assay (ChIP)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	6D3
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_1907437

Product Specific Information

Description: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection.

Applications Reported: This 6D3 antibody has been reported for use in immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections.

Applications Tested: This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blottiing, this antibody can be used at 1-10 µg/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale.

PeerJ

Wang X,Liao T,Wan C,Yang X,Zhao J,Fu R,Yao Z,Huang Y,Shi Y,Chang G,Zheng Y,Luo F,Liu Z,Wang Y,Mao X,Zhao XY

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Immunocytochemistry

Thu Mar 31 00:00:00 EDT 2022

Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.

Theranostics

Ge W,Tan SJ,Wang SH,Li L,Sun XF,Shen W,Wang X

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Immunohistochemistry

Mon May 03 00:00:00 EDT 2021

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

14-5963-82 was used in Immunohistochemistry (Paraffin) to show that depletion of MCPIP1 in macrophages and granulocytes (Mcpip1fl/fl-LysMcre+ C57BL/6 mice) is sufficient to trigger severe autoimmune disease.

Thu Mar 18 00:00:00 EDT 2021

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

Thu Mar 18 00:00:00 EDT 2021

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

Thu Mar 18 00:00:00 EDT 2021

MEF2D sustains activation of effector Foxp3+ Tregs during transplant survival and anticancer immunity.

The Journal of clinical investigation

Di Giorgio E,Wang L,Xiong Y,Akimova T,Christensen LM,Han R,Samanta A,Trevisanut M,Bhatti TR,Beier UH,Hancock WW

14-5963 was used in Western Blotting to study the role played by MEF2D in sustaining effector Foxp3+ Treg functions without abrogating their basal actions suggests its suitability for drug discovery efforts in cancer therapy.

Tue Dec 01 00:00:00 EST 2020

Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesis.

Nature communications

Oulès B,Philippeos C,Segal J,Tihy M,Vietri Rudan M,Cujba AM,Grange PA,Quist S,Natsuga K,Deschamps L,Dupin N,Donati G,Watt FM

14-5963-82 was used in Immunohistochemistry (Paraffin) to show that GATA6, which is expressed in the upper pilosebaceous unit of normal human skin, is down-regulated in acne.

Tue Oct 20 00:00:00 EDT 2020

Principles of early human development and germ cell program from conserved model systems.

Nature

Kobayashi T,Zhang H,Tang WWC,Irie N,Withey S,Klisch D,Sybirna A,Dietmann S,Contreras DA,Webb R,Allegrucci C,Alberio R,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Principles of early human development and germ cell program from conserved model systems.

Nature

Kobayashi T,Zhang H,Tang WWC,Irie N,Withey S,Klisch D,Sybirna A,Dietmann S,Contreras DA,Webb R,Allegrucci C,Alberio R,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Principles of early human development and germ cell program from conserved model systems.

Nature

Kobayashi T,Zhang H,Tang WWC,Irie N,Withey S,Klisch D,Sybirna A,Dietmann S,Contreras DA,Webb R,Allegrucci C,Alberio R,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

The aryl hydrocarbon receptor controls cell-fate decisions in B cells.

The Journal of experimental medicine

Vaidyanathan B,Chaudhry A,Yewdell WT,Angeletti D,Yen WF,Wheatley AK,Bradfield CA,McDermott AB,Yewdell JW,Rudensky AY,Chaudhuri J

14-5963 was used in Western Blotting to show that the aryl hydrocarbon receptor is highly induced upon B cell activation and serves a critical role in regulating activation-induced cell fate outcomes.

Sun Jan 01 00:00:00 EST 2017

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

14-5963 was used in Immunofluorescence to investigate the role of nanog in unipotent primordial germ cells in mice.

Thu Jan 21 00:00:00 EST 2016

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

14-5963 was used in Immunofluorescence to investigate the role of nanog in unipotent primordial germ cells in mice.

Thu Jan 21 00:00:00 EST 2016

Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.

PLoS genetics

Mould AW,Morgan MA,Nelson AC,Bikoff EK,Robertson EJ

14-5963 was used in Immunohistochemistry to demonstrate that Blimp1/Prdm1 is pivotal in the postnatal metabolic switch that occurs in the small intestine.

Wed Jul 01 00:00:00 EDT 2015

Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.

PLoS genetics

Mould AW,Morgan MA,Nelson AC,Bikoff EK,Robertson EJ

14-5963 was used in Immunohistochemistry to demonstrate that Blimp1/Prdm1 is pivotal in the postnatal metabolic switch that occurs in the small intestine.

Wed Jul 01 00:00:00 EDT 2015

Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.

PLoS genetics

Mould AW,Morgan MA,Nelson AC,Bikoff EK,Robertson EJ

14-5963 was used in Immunohistochemistry to demonstrate that Blimp1/Prdm1 is pivotal in the postnatal metabolic switch that occurs in the small intestine.

Wed Jul 01 00:00:00 EDT 2015

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

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Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Western Blot

Wed Apr 01 00:00:00 EDT 2015

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

14-5963 was used in Immunofluorescence to demonstrate the specification of human primordial germ cell-like cells from germline competent pluripotent stem cells.

Thu Jan 15 00:00:00 EST 2015

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

14-5963 was used in Immunofluorescence to demonstrate the specification of human primordial germ cell-like cells from germline competent pluripotent stem cells.

Thu Jan 15 00:00:00 EST 2015

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

14-5963 was used in Immunofluorescence to demonstrate the specification of human primordial germ cell-like cells from germline competent pluripotent stem cells.

Thu Jan 15 00:00:00 EST 2015

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

14-5963 was used in Immunofluorescence to demonstrate the specification of human primordial germ cell-like cells from germline competent pluripotent stem cells.

Thu Jan 15 00:00:00 EST 2015

Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses.

Journal of immunology (Baltimore, Md. : 1950)

White CA,Pone EJ,Lam T,Tat C,Hayama KL,Li G,Zan H,Casali P

14-5963 was used in Western Blotting to study the epigenetic mechanisms which modulate expression of proteins involved in B cell differentiation and autoimmunity..

Mon Dec 15 00:00:00 EST 2014

Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.

Journal of immunology (Baltimore, Md. : 1950)

Park KS,Bayles I,Szlachta-McGinn A,Paul J,Boiko J,Santos P,Liu J,Wang Z,Borghesi L,Milcarek C

14-5963 was used in Western Blotting to define the effects of the transcription elongation factor eleven nineteen lysine-rich leukaemia gene.

Sat Nov 01 00:00:00 EDT 2014

BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.

Stem cell reports

Kretzschmar K,Cottle DL,Donati G,Chiang MF,Quist SR,Gollnick HP,Natsuga K,Lin KI,Watt FM

Tue Oct 14 00:00:00 EDT 2014

A genome-wide regulatory network identifies key transcription factors for memory CD8⁺ T-cell development.

Nature communications

Hu G,Chen J

14-5963 was used in Western Blotting to investigate the transcriptional control of memory CD8+ T-cell development.

Tue Jul 15 00:00:00 EDT 2014

Distinct fibroblast lineages determine dermal architecture in skin development and repair.

Nature

Driskell RR,Lichtenberger BM,Hoste E,Kretzschmar K,Simons BD,Charalambous M,Ferron SR,Herault Y,Pavlovic G,Ferguson-Smith AC,Watt FM

14-5963 was used in Immunohistochemistry to explain why wounding is linked to formation of ECM-rich scar tissue that lacks hair follicles.

Thu Dec 12 00:00:00 EST 2013

Sox2 modulates the function of two distinct cell lineages in mouse skin.

Developmental biology

Lesko MH,Driskell RR,Kretzschmar K,Goldie SJ,Watt FM

14-5963 was used in Immunohistochemistry to investigate how Sox2 modulates the function of two distinct cell lineages in mouse skin.

Tue Oct 01 00:00:00 EDT 2013

Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation.

EMBO reports

Grabole N,Tischler J,Hackett JA,Kim S,Tang F,Leitch HG,Magnúsdóttir E,Surani MA

Mon Jul 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; RP1-134E15.1

Gene Aliases: b2b1765Clo; Blimp-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1

UniProt ID: (Human) O75626, (Mouse) Q60636

Entrez Gene ID: (Human) 639, (Mouse) 12142

Molecular Function: C2H2 zinc finger transcription factor