|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (363 CVSHNASPPTSSSQYPS 378) of Brachyury protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Brachyury is a 435 amino acid containing embryonic nuclear transcription factor binding to the palindromic T-site. It binds to the T-box in the N-terminus to regulate the genes required for mesodermal differentiation. It is the earliest indicator of mesoderm formation and differentiation. Brachyury is expressed during gastrula and neurula stages in involuting mesoderm and in the notochord.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.