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Invitrogen

Brachyury Polyclonal Antibody

View all (9) Brachyury antibodies
635D49A6-4B57-425E-8DC4-6BA1AEB7B473 Created with sketchtool. Datasheet
433BB748-DC81-4FE4-83AD-384F97313745 Created with sketchtool. Create Citation
C488C313-B559-4BFD-B17D-A8B65492815A Created with sketchtool. Tech Support
635D49A6-4B57-425E-8DC4-6BA1AEB7B473 Created with sketchtool. Datasheet
433BB748-DC81-4FE4-83AD-384F97313745 Created with sketchtool. Create Citation
C488C313-B559-4BFD-B17D-A8B65492815A Created with sketchtool. Tech Support

Product Details

Tested Applications
Dilution
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1000
Product Specifications
Species Reactivity
Human, Mouse, Rat
Host / Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the centers region of Human Brachyury. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.82 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2735533

Product Specific Information

Predicted Reactivity: Human (100%), Mouse (84%), Dog (82%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Brachyury encodes a protein that is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: brachyury homolog; Brachyury protein; brachyury-like 2; brachyury-like 3; low ratio; Protein T; T; T brachyury homolog; T brachyury transcription factor; T, brachyury homolog; T-box transcription factor T; TFT

Gene Aliases: Bra; cou; D17Mit170; Low; Lr; me75; SAVA; T; T1; TBXT; TFT; Tl2; Tl3

UniProt ID: (Human) O15178, (Mouse) P20293

Entrez Gene ID: (Human) 6862, (Rat) 292301, (Mouse) 20997

Molecular Function: nucleic acid binding transcription factor

Function(s)
negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter anatomical structure morphogenesis anterior/posterior pattern specification reproductive process notochord development positive regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated multicellular organism development cardiac muscle cell differentiation
Process(es)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function sequence-specific DNA binding DNA binding protein binding

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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