Invitrogen

Brachyury Polyclonal Antibody

View all (17) Brachyury antibodies

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Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Brachyury in paraffin-embedded rat testis. Sample was incubated with Brachyury polyclonal antibody (Product # PA5-78421) using a dilution of 1:2000.

Product Details

PA5-78421

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the centers region of Human Brachyury. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	1.08 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735533

Product Specific Information

Predicted Reactivity: Human (100%), Mouse (84%), Dog (82%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: brachyury homolog; Brachyury protein; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T

Gene Aliases: SAVA; T; TBXT; TFT

UniProt ID: (Human) O15178

Entrez Gene ID: (Human) 6862, (Rat) 292301

Molecular Function: Rel homology transcription factor

negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter anatomical structure morphogenesis anterior/posterior pattern specification reproductive process notochord development positive regulation of transcription from RNA polymerase II promoter

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function sequence-specific DNA binding