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|Tested species reactivity||Chem|
|Published species reactivity||Mouse, Rhesus monkey, Not Applicable|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fgf10 is required for specification of non-sensory regions of the cochlear epithelium.
B35128 was used in immunohistochemistry to investigate the role of Fgf10 in the development of the vertebrate inner ear
|Urness LD,Wang X,Shibata S,Ohyama T,Mansour SL||Developmental biology (400:59)||2015|
|Not Applicable||Not Cited||
A bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos.
B35128 was used in immunohistochemistry to describe a method to use BrdU incorporation in Xenopus embryos.
|Auger H,Thuret R,El Yakoubi W,Papalopulu N||Methods in molecular biology (Clifton, N.J.) (917:461)||2012|
|Rhesus monkey||Not Cited||
Expression of the mannose receptor CD206 in HIV and SIV encephalitis: a phenotypic switch of brain perivascular macrophages with virus infection.
B35128 was used in flow cytometry and immunohistochemistry to examine the diagnostic value of mannose receptor CD206 in HIV and simian immunodeficiency virus encephalitis
|Holder GE,McGary CM,Johnson EM,Zheng R,John VT,Sugimoto C,Kuroda MJ,Kim WK||Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology (9:716)||2014|
Bone marrow stromal cells enhance the osteogenic properties of hydroxyapatite scaffolds by modulating the foreign body reaction.
B35128 was used in immunohistochemistry - paraffin section to study the osteogenic properties of bone marrow stromal cell-loaded biomimetic constructs and assess the cellular components elicited
|Tour G,Wendel M,Tcacencu I||Journal of tissue engineering and regenerative medicine (8:841)||2014|