|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (IHC)||See 3 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Tested Species reactivity||Chemical|
|Published species reactivity||Mouse , Rhesus monkey|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage conditions||4° C|
The MoBU-1 clone has a high specificity for BrdU, in addition to no cross-reactivity with EdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Samples labeled with the Alexa Fluor™ conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging. The unconjugated form and the biotin conjugate of this clone require a secondary antibody or a streptavidin conjugate for detection, respectively. The MoBU-1 clone is also available for flow cytometry applications.
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic nucleoside that is an analog of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat. Although this method is rapidly being replaced with the recently developed click chemistry-based Click-iT™ EdU assay, an advantage of the antibody clone MoBU-1 is that it does not cross-react with the thymidine analog EdU (5-ethynyl-2'-deoxyuridine), thus making it valuable for use in dual-pulse applications. Traditionally, the dual-pulse method, which is used for studying biological events such as neurogenesis and cell differentiation or evaluating anti-cancer drug efficacy, employs BrdU immunocytochemistry with iododeoxyuridine (IdU) or chlorodeoxyuridine (CldU), using multiple antibodies for detection. However, using sequential pulses of the thymidine analogs EdU and BrdU greatly simplifies dual-pulse labeling, and allows a straightforward and reliable method of distinguishing BrdU- and EdU-labeled cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
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