Invitrogen

C1QA Polyclonal Antibody

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C1QA Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of C1q was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: C1q Polyclonal Antibody (Product # PA5-29586) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

C1QA Antibody in Western Blot (WB) — Western blot analysis of C1q was performed by separating 30 µg of human plasma by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a C1q Polyclonal Antibody (Product # PA5-29586) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-29586

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 13 and 225 of Human Complement C1qA View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.96 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547062

Product Specific Information

Recommended positive controls: human plasma, mouse spleen.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits.

Glia

Farfara D,Feierman E,Richards A,Revenko AS,MacLeod RA,Norris EH,Strickland S

PA5-29586 was used in Western Blotting to highlight the important role of regulated plasma C1INH as it acts as a gatekeeper to the brain via the neurovascular system.

Mon Jul 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: complement C1q chain A; Complement C1q subcomponent subunit A; complement component 1, q subcomponent, A chain; complement component 1, q subcomponent, alpha polypeptide; complement component C1q, A chain

Gene Aliases: AI255395; C1q; C1QA

UniProt ID: (Human) P02745, (Mouse) P98086

Entrez Gene ID: (Human) 712, (Mouse) 12259

Function(s)

proteolysis complement activation complement activation, classical pathway cell-cell signaling response to iron ion innate immune response immune system process

Process(es)

serine-type endopeptidase activity protein binding