|Tested species reactivity||Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat|
|Published species reactivity||Guinea pig|
|Host / Isotype||Goat / IgG|
|Immunogen||Human complement C3|
|Storage buffer||TBS, pH 7.5|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunoelectrophoresis (IE)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-29715 detects C3 from human, equine, hamster, Guinea pig, mouse, rabbit, rat, pig, and non-human primate samples. PA1-29715 does not cross react wtih bovine, canine, chicken, feline and sheep samples.
PA1-29715 has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications.
The PA1-29715 immunogen is Human complement C3.
The complement system provides innate defense against microbial infection and is a "complement" to antibody mediated immunity. The complement system consists of thirty five interacting plasma and membrane associated proteins which contribute to host-defense and initiate and amplify inflammation, even in the preimmune state where specific antibodies and lymphocytes are not available. In addition to the complement components themselves, this system also contains several soluble factors that prevent spontaneous complement activation from occurring in solution, as well as, several regulatory membrane associated proteins that protect host cells from accidental complement attack. Cleavage of the C3 component by either the classical pathway or the alternative pathway releases C3a and C3b. C3a appears to be important in many inflammatory responses while the C3b fragment covalently binds to the cell or bacterial surface and plays a role in opsonisation. Binding of C3b to the C4b component of the C3 convertase, results in C5 convertase (C4b3b2a) formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The immunosuppression mechanism of hypodermin A on complement.
PA1-29715 was used in western blot to study the mechanism by which hypodermin A inhibits the complement pathway
|Chen RJ,Hu AK,Yuan HH,Wang ZZ,Ji DJ,Wu LL,Zhang TY,Zhu YH,Sun W,Zhu XR||Parasitology international (63:392)||2014|