Bethyl Laboratories

CARM1 Polyclonal Antibody

View all (21) PRMT4 antibodies

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CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Product # A300-421A; lot 4) used at 1:1,000 (1 µg/mL). Detection: DAB.

CARM1 Antibody in Immunohistochemistry (IHC) — Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Cat. No. A300-421A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

CARM1 Antibody in Western Blot (WB) — Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody A300-421A (lot A300-421A-3) used for IP at 6 µg per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (A300-421A-2) and rabbit anti-CARM1 antibody A300-420A. For blotting immunoprecipitated CARM1, A300-421A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

CARM1 Antibody in Western Blot (WB) — Detection of human and mouse CARM1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody A300-421A (lot A300-421A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

CARM1 Antibody in Western Blot (WB) — Detection of human CARM1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody (Product # A300-421A; lot A300-421A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

CARM1 Antibody in Western Blot (WB) — Detection of mouse CARM1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody (Product # A300-421A; lot A300-421A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

CARM1 Antibody in Immunoprecipitation (IP) — Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody (Product # A300-421A; lot A300-421A-4) used for IP at 6 µg per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (lot A300-421A-3) and rabbit anti-CARM1 antibody (Product # A300-420A). For blotting immunoprecipitated CARM1 (Product # A300-421A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Product Details

A300-421A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:10,000-1:25,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500 to 1:2,000	-
Immunoprecipitation (IP)	2-10 µg/mg lysate	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 550 and C-term
Conjugate	Unconjugated
Form	liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	tris citrate/phosphate, pH 7-8
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Rat

Target Information

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Coactivator-associated arginine methyltransferase 1; Histone-arginine methyltransferase CARM1; protein arginine methyltransferase; Protein arginine N-methyltransferase 4

Gene Aliases: CARM1; PRMT4

UniProt ID: (Human) Q86X55, (Mouse) Q9WVG6

Entrez Gene ID: (Human) 10498, (Mouse) 59035

Molecular Function: protein modifying enzyme

regulation of growth plate cartilage chondrocyte proliferation transcription, DNA-templated regulation of transcription, DNA-templated DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest aging positive regulation of cell proliferation viral process histone methylation peptidyl-arginine methylation, to asymmetrical-dimethyl arginine intracellular estrogen receptor signaling pathway negative regulation of protein binding regulation of intracellular estrogen receptor signaling pathway histone H3-R2 methylation histone H3-R17 methylation cellular lipid metabolic process positive regulation of fat cell differentiation response to cAMP endochondral bone morphogenesis regulation of mRNA binding negative regulation of dendrite development protein methylation pathogenesis chromatin modification intracellular steroid hormone receptor signaling pathway methylation histone arginine methylation peptidyl-arginine N-methylation

Process(es)

transcription coactivator activity protein binding beta-catenin binding protein methyltransferase activity histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity ligand-dependent nuclear receptor transcription coactivator activity protein-arginine omega-N asymmetric methyltransferase activity histone methyltransferase activity (H3-R17 specific) histone methyltransferase activity protein homodimerization activity transcription regulatory region DNA binding lysine-acetylated histone binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity