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Invitrogen
CASPASE 1 P10 (CLEAVED ALA317) Polyclonal Antibody
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FIGURE: 1 / 8
CASPASE 1 P10 (CLEAVED ALA317) Antibody (PA5-105049) in ICC/IF
Immunofluorescent analysis of Caspase 1 p10 (Cleaved Arg317) in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Caspase 1 p10 (Cleaved Arg317) polyclonal antibody (Product # PA5-105049) using a dilution of 1:100 (primary, 1 hr, 37°C), 1:200 (tubulin), and followed by goat anti-mous... View More
Product Details
PA5-105049
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CASP1(Accession P29466), corresponding to amino acid residues T310-S332.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Ala317.
Target Information
Caspase-1 p10 (cleaved Ala317) refers to the small, active subunit generated following proteolytic processing of pro-caspase-1, an inflammatory cysteine protease encoded by the CASP1 gene. Caspase-1 is synthesized as an inactive zymogen that becomes activated within multiprotein inflammasome complexes in response to pathogenic infection or cellular stress. Activation involves autocatalytic cleavage at specific aspartic acid residues, including near alanine 317, resulting in the formation of the p20 (large) and p10 (small) subunits that assemble into the catalytically active enzyme. The cleaved p10 fragment is therefore a marker of caspase-1 activation. Active caspase-1 mediates the maturation and secretion of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-18 (IL-18), and plays a central role in pyroptosis, a form of inflammatory programmed cell death. Detection of cleaved caspase-1 p10 (Ala317) is widely used as an indicator of inflammasome activation in immunology and inflammation research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Active Caspase-1 (p10); CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1; Caspase-1 (cleaved); Cleaved Caspase-1; cysteine protease; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45; unnamed protein product
Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45
UniProt ID: (Human) P29466, (Mouse) P29452
Entrez Gene ID: (Human) 834, (Mouse) 12362, (Rat) 25166
endopeptidase activity
cysteine-type endopeptidase activity
protein binding
peptidase activity
cysteine-type peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
hydrolase activity
enzyme binding
kinase binding
cytokine binding
identical protein binding
CARD domain binding
scaffold protein binding
protease
negative regulation of transcription from RNA polymerase II promoter
plasma membrane repair
pattern recognition receptor signaling pathway
proteolysis
apoptotic process
inflammatory response
signal transduction
osmosensory signaling pathway
protein secretion
protein autoprocessing
protein catabolic process
response to lipopolysaccharide
positive regulation of interleukin-1 beta production
positive regulation of interleukin-18 production
interleukin-18-mediated signaling pathway
defense response to bacterium
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
icosanoid biosynthetic process
ceramide biosynthetic process
regulation of inflammatory response
positive regulation of inflammatory response
protein maturation
defense response to virus
protein poly-ADP-ribosylation
pyroptosis
interleukin-1-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular response to interferon-gamma
cytokine precursor processing
signaling receptor ligand precursor processing
AIM2 inflammasome complex assembly
pyroptotic cell death
positive regulation of tumor necrosis factor-mediated signaling pathway
response to hypoxia
microglial cell activation
myoblast fusion
memory
response to bacterium
regulation of autophagy
response to organic cyclic compound
protein processing
interleukin-1 beta production
response to ATP
response to drug
positive regulation of apoptotic process
positive regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of cytokine secretion
positive regulation of interleukin-1 alpha secretion
positive regulation of interleukin-1 beta secretion
mitochondrial depolarization
membrane hyperpolarization
cellular response to organic substance
programmed necrotic cell death
toxin transport
midgut development
response to toxic substance
lung development
execution phase of apoptosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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