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Invitrogen
CCL21 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CCL21 Antibody (500-P109BT-25UG) in WB
Western Blot: To detect hExodus-2 by Western Blot analysis CCL21 Polyclonal Antibody, Biotin (Product # 500-P109BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P109BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Exodus-2 (CCL21)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: discontinued.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Exodus-2 (CCL21). Anti-Human Exodus-2 (CCL21)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Exodus-2 (CCL21) (500-P109) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Exodus-2 (CCL21).
Western Blot: To detect hExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P109BT-1MG will be provided as 2 x 500 µg
Target Information
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6 Ckine; 6Ckine; 6Ckine; CC chemokine; beta chemokine exodus-2; Beta-chemokine exodus-2; C-C motif chemokine 21; CCL21; chemokine (C-C motif) ligand 21; Efficient Chemoattractant for Lymphocytes; secondary lymphoid tissue chemokine; Secondary lymphoid-tissue chemokine; SLC; small inducible cytokine subfamily A (Cys-Cys), member 21; Small-inducible cytokine A21; unnamed protein product
Gene Aliases: 6Ckine; CCL21; CKb9; ECL; SCYA21; SLC; TCA4; UNQ784/PRO1600
UniProt ID: (Human) O00585
Entrez Gene ID: (Human) 6366
establishment of T cell polarity
immunological synapse formation
positive regulation of cell-matrix adhesion
dendritic cell chemotaxis
positive regulation of dendritic cell antigen processing and presentation
chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of glycoprotein biosynthetic process
positive regulation of T cell chemotaxis
cell migration
positive regulation of cell migration
positive regulation of actin filament polymerization
positive regulation of pseudopodium assembly
T cell costimulation
ruffle organization
killing of cells of other organism
positive regulation of cell adhesion mediated by integrin
mesangial cell-matrix adhesion
chemokine (C-C motif) ligand 21 signaling pathway
CCL21-activated CCR7 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of JNK cascade
eosinophil chemotaxis
positive regulation of receptor-mediated endocytosis
cell maturation
positive regulation of chemotaxis
release of sequestered calcium ion into cytosol
positive regulation of filopodium assembly
positive regulation of protein kinase B signaling
cell chemotaxis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to prostaglandin E stimulus
positive regulation of neutrophil chemotaxis
dendritic cell dendrite assembly
positive regulation of phospholipase C/protein kinase C signal transduction
negative regulation of leukocyte tethering or rolling
cellular response to chemokine
positive regulation of cell motility
positive regulation of T cell migration
positive regulation of myeloid dendritic cell chemotaxis
negative regulation of dendritic cell dendrite assembly
negative regulation of dendritic cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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