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Invitrogen
CCL3 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 4
CCL3 Antibody (500-P77-1MG) in WB
Western Blot: To detect Rat MIP-1alpha by Western Blot analysis CCL3 Polyclonal Antibody (Product # 500-P77-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P77-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat MIP-1alpha (CCL3)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
1 mg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MIP-1alpha (CCL3). Anti-Rat MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography employing an immobilized Rat MIP-1alpha (CCL3) matrix.
Sandwich ELISA: To detect Rat MIP-1alpha (CCL3) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat MIP-1alpha (CCL3) (500-P77Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MIP-1alpha (CCL3).
Western Blot: To detect Rat MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 3; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; RP23-320E6.7; small inducible cytokine A3; Small-inducible cytokine A3
Gene Aliases: Ccl3; MIP-1a; Mip1a; Scya3
UniProt ID: (Rat) P50229
Entrez Gene ID: (Rat) 25542
protein kinase activity
calcium-dependent protein kinase C activity
chemokine activity
heparin binding
phospholipase activator activity
kinase activity
CCR1 chemokine receptor binding
CCR5 chemokine receptor binding
chemoattractant activity
identical protein binding
cytokine
intercellular signal molecule
MAPK cascade
osteoblast differentiation
cell activation
monocyte chemotaxis
protein phosphorylation
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
cytoskeleton organization
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
regulation of cell shape
response to toxic substance
positive regulation of gene expression
negative regulation of gene expression
T cell chemotaxis
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
calcium-mediated signaling
signaling
positive regulation of cell migration
negative regulation of bone mineralization
neutrophil chemotaxis
leukocyte chemotaxis
lipopolysaccharide-mediated signaling pathway
positive regulation of tumor necrosis factor production
response to drug
positive regulation of catalytic activity
eosinophil degranulation
protein kinase B signaling
positive regulation of neuron apoptotic process
astrocyte cell migration
negative regulation by host of viral transcription
negative regulation of osteoclast differentiation
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of interleukin-1 beta secretion
positive regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
regulation of sensory perception of pain
cell chemotaxis
positive regulation of ERK1 and ERK2 cascade
response to cholesterol
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to organic cyclic compound
granulocyte chemotaxis
positive regulation of natural killer cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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