Invitrogen

CCL5 (RANTES) Monoclonal Antibody (VL1)

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CCL5 (RANTES) Antibody in ELISA (ELISA) — Fig. 1 Ccl5 expression from T cells is repressed by RUNX/CBFbeta complexes. a Expression profiles assessed by ELISA of CCL3, CCL4, and CCL5 and the selected cytokines IL-3, IL-4, and IFNgamma in supernatants of in vitro-stimulated CD4 + and CD8 + T cells at 5 days after stimulation. A summary of three independent measurements on three mice (with their genotypes indicated) are shown. Error bars indicate Mean +- SD and each dot represents a mouse examined over at least two independent experiments. Statistical significance is measured via unpaired two-tailed Student's t tests and is presented as follows: * p < 0.05, ** p < 0.01. b Flow cytometry analysis of total splenocytes and splenic TCRbeta + T cells in mice with their genotypes indicated. One representative profile of the CCL5 expression is shown in the histograms and dot plots. Numbers in the dot plots indicate the percentage of cells in each quadrant. Error bars indicate Mean +- SD and each dot represents a mouse examined over at least two independent experiments. Statistical significance is measured via unpaired two-tailed Student's t tests and is presented as follows: ** p < 0.01, **** p < 0.0001. Source data are provided as a Source Data file.

Product Details

AHC1052

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

VL1

Immunogen

Recombinant human RANTES.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2536267

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity.

Nature communications

Seo W,Shimizu K,Kojo S,Okeke A,Kohwi-Shigematsu T,Fujii SI,Taniuchi I

AHC1052 was used in ELISA to suggest that RUNX3-mediated CCL5 repression is critical for modulating anti-tumor immunity.

Thu Mar 26 00:00:00 EDT 2020

Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity.

Nature communications

Seo W,Shimizu K,Kojo S,Okeke A,Kohwi-Shigematsu T,Fujii SI,Taniuchi I

AHC1052 was used in ELISA to suggest that RUNX3-mediated CCL5 repression is critical for modulating anti-tumor immunity.

Thu Mar 26 00:00:00 EDT 2020

Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands.

Proceedings of the National Academy of Sciences of the United States of America

Lu Y,Xue Q,Eisele MR,Sulistijo ES,Brower K,Han L,Amir el-AD,Pe'er D,Miller-Jensen K,Fan R

AHC1052 was used in dot blot to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage

Tue Feb 17 00:00:00 EST 2015

Dose dependent effects of platelet derived chondroitinsulfate A on the binding of CCL5 to endothelial cells.

BMC immunology

Weingart C,Nelson PJ,Krämer BK,Mack M

AHC1052 was used in flow cytometry to assess the impact of platelet derived glycosaminoglycans on the immobilization of CCL5 on human microvascular endothelial cells

Wed Dec 10 00:00:00 EST 2008

Oral biopsies from patients with orofacial granulomatosis with histology resembling Crohn's disease have a prominent Th1 environment.

Inflammatory bowel diseases

Freysdottir J,Zhang S,Tilakaratne WM,Fortune F

AHC1052 was used in immunohistochemistry - paraffin section to discuss if patients with Crohn's disease and orofacial granulomatosis should be considered as a unique subset

Sun Apr 01 00:00:00 EDT 2007

Platelet microparticles: a transcellular delivery system for RANTES promoting monocyte recruitment on endothelium.

Arteriosclerosis, thrombosis, and vascular biology

Mause SF,von Hundelshausen P,Zernecke A,Koenen RR,Weber C

AHC1052 was used in immunocytochemistry and western blot to study the role of platelet microparticles in delivering RANTES and promoting monocyte recruitment

Fri Jul 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; RANTES(3-68); RANTES(4-68); regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

Gene Aliases: CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228

UniProt ID: (Human) P13501

Entrez Gene ID: (Human) 6352

Molecular Function: cytokine intercellular signal molecule

Function(s)

Process(es)

phosphatidylinositol phospholipase C activity protein kinase activity protein binding chemokine activity heparin binding phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR4 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding protein homodimerization activity protein self-association chemokine receptor antagonist activity

CCL5 (RANTES) Monoclonal Antibody (VL1)

Product Details

AHC1052

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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