Invitrogen

CCP2 Polyclonal Antibody

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CCP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CCP2 in HUVEC cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with CCP2 polyclonal antibody (Product # PA5-101547) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

CCP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded CCP2 in human prostate tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CCP2 polyclonal antibody (Product # PA5-101547) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

CCP2 Antibody in Western Blot (WB) — Western blot analysis of CCP2 in HUVEC cells. Samples were incubated with CCP2 polyclonal antibody (Product # PA5-101547).

Product Details

PA5-101547

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human CBCP2, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2850981

Target Information

Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP/GTP binding protein-like 2; ATP/GTP-binding protein-like 2; carboxypeptidase 2, cytosolic; CBCP2; cytoplasmic carboxypeptidase 2; Cytosolic carboxypeptidase 2; testis tissue sperm-binding protein Li 96mP

Gene Aliases: 4930524K04; A430081C19Rik; AGBL2; CCP2

UniProt ID: (Human) Q5U5Z8, (Mouse) Q8CDK2

Entrez Gene ID: (Human) 79841, (Mouse) 271813

Molecular Function: metalloprotease