|Flow Cytometry (Flow)||0.25 µg/test|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The OX62 monoclonal antibody reacts with rat CD103, also known as alphaE2 integrin. CD103 was originally identified on rat veiled dendritic cells, and is expressed by dendritic epidermal T cells in the skin, intraepithelial lymphocytes in the small intestine and by dendritic cells associated with gammadelta T cells. Integrins are a family of heterodimeric proteins which contain non-covalently associated alpha and beta transmembrane glycoproteins. Integrins function as receptors for extracellular matrix and cell-cell adhesion molecules.
Applications Reported: This OX62 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX62 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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