Invitrogen

CD105 (Endoglin) Monoclonal Antibody (MJ7/18), Alexa Fluor™ 488, eBioscience™

Product Image Gallery

CD105 (Endoglin) Antibody in Flow Cytometry (Flow) — Staining of bEnd-3 cell line with 0.5 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (blue histogram) or 0.5 µg of CD105 (Endoglin) Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

CD105 (Endoglin) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Immunocytochemistry

CD105 (Endoglin) Antibody in Immunohistochemistry (IHC) — FIG. 4. Cluster of differentiation 34 (CD34) costains with CD105. Maximum intensity projection images of mouse inguinal fat pad, human abdominal fat, and human gluteofemoral adipose tissue stained with CD34, Hoescht, and the ASC marker CD105. Merged images demonstrate a high degree of overlap between CD34 and CD105 antibody binding. ASC, adipose-derived stromal cells.

CD105 (Endoglin) Antibody in Immunohistochemistry (PFA fixed), Flow Cytometry (IHC (PFA), Flow) — Fig 4 Specificity of LysMCre-mediated lox P recombination in MPhi. (A) Confocal microscopy of liver sections from C56BL/6 mice. Endoglin (green) is highly expressed on hepatic sinusoids (arrows) and on endothelium from hepatic vein (V) in Eng wt/wt LysMCre and Eng fl/fl LysMCre . (B) sEng levels in serum samples. Levels of sEng in serum from Eng wt/wt LysMCre (n = 14), Eng wt/fl LysMCre (n = 7) and Eng fl/fl LysMCre mice (n = 14) were determined by ELISA. The mean value of sEng in each group is shown in the upper part. Horizontal line represents the median. ANOVA with Bonferroni''s post-test was performed and no statistical differences were observed. (C) qRT-PCR for Eng mRNA expression in PerC MPhi. 18s was used as a reference gene, and data were normalized to Eng wt/wt LysMCre mice. Data are presented as the mean +- SEM and are pooled from five independent experiments (N = 5) with each performed in triplicate; Data were analyzed versus respective controls using Student''s t test. **P<0.01 and ***P<0.001. (D) Endoglin expression levels in resident PerC MPhi from experimental animals. Flow cytometry plots depict the percentage of endoglin-positive cells among total peritoneal resident MPhi (F4/80 pos , mainly LPM) in Eng wt/wt LysMCre , Eng wt/fl LysMCre and Eng fl/fl LysMCre mice.

Product Details

53-1051-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1.0 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MJ7/18

Conjugate

Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2815204

Product Specific Information

Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: This MJ7/18 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Extracellular vesicles from recombinant cell factories improve the activity and efficacy of enzymes defective in lysosomal storage disorders.

Journal of extracellular vesicles

Seras-Franzoso J,Díaz-Riascos ZV,Corchero JL,González P,García-Aranda N,Mandaña M,Riera R,Boullosa A,Mancilla S,Grayston A,Moltó-Abad M,Garcia-Fruitós E,Mendoza R,Pintos-Morell G,Albertazzi L,Rosell A,Casas J,Villaverde A,Schwartz S,Abasolo I

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Induction of mesenchymal stem cell‑like transformation in rat primary glial cells using hypoxia, mild hypothermia and growth factors.

Molecular medicine reports

Wei H,Zhou W,Hu G,Shi C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

The microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression.

Nature communications

Huang J,Zhao L,Fan Y,Liao L,Ma PX,Xiao G,Chen D

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Fri Jun 28 00:00:00 EDT 2019

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms.

The Journal of clinical investigation

Stivala S,Codilupi T,Brkic S,Baerenwaldt A,Ghosh N,Hao-Shen H,Dirnhofer S,Dettmer MS,Simillion C,Kaufmann BA,Chiu S,Keller M,Kleppe M,Hilpert M,Buser AS,Passweg JR,Radimerski T,Skoda RC,Levine RL,Meyer SC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Mon Mar 04 00:00:00 EST 2019

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Immunohistochemistry

Mon Oct 01 00:00:00 EDT 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Immunohistochemistry

Mon Oct 01 00:00:00 EDT 2018

RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells.

The Journal of biological chemistry

Ge J,Burnier L,Adamopoulou M,Kwa MQ,Schaks M,Rottner K,Brakebusch C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

Isolation and functional assessment of mouse skeletal stem cell lineage.

Nature protocols

Gulati GS,Murphy MP,Marecic O,Lopez M,Brewer RE,Koepke LS,Manjunath A,Ransom RC,Salhotra A,Weissman IL,Longaker MT,Chan CKF

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Cardiosphere-Derived Cells Require Endoglin for Paracrine-Mediated Angiogenesis.

Stem cell reports

Redgrave RE,Tual-Chalot S,Davison BJ,Singh E,Hall D,Amirrasouli MM,Gilchrist D,Medvinsky A,Arthur HM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Tue May 09 00:00:00 EDT 2017

Mice Lacking Endoglin in Macrophages Show an Impaired Immune Response.

PLoS genetics

Ojeda-Fernández L,Recio-Poveda L,Aristorena M,Lastres P,Blanco FJ,Sanz-Rodríguez F,Gallardo-Vara E,de las Casas-Engel M,Corbí Á,Arthur HM,Bernabeu C,Botella LM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 53-1051-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Mice Lacking Endoglin in Macrophages Show an Impaired Immune Response.

PLoS genetics

Ojeda-Fernández L,Recio-Poveda L,Aristorena M,Lastres P,Blanco FJ,Sanz-Rodríguez F,Gallardo-Vara E,de las Casas-Engel M,Corbí Á,Arthur HM,Bernabeu C,Botella LM