|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse intestinal APN|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. This clone has been reported to block mouse APN enzyme activity.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
CD13 cell surface glycoprotein is a 150kDa molecule expressed by granulocytes and monocytes, and by myeloid leukemia cells. CD13 functions as an aminopeptidase enzyme and is also alpha receptor for coronavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.