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Invitrogen
CD171 Monoclonal Antibody (eBio5G3 (5G3)), PE, eBioscience™
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CD171 Antibody (12-1719-42) in Flow
Knockout of CD171 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR651984_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD171 was performed by staining HeLa CD171 Knock out cells with 0.25 µg Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ (Product # 12-4724-82, yellow histogram) or 0.25 µg CD171 Monoclonal Antibody (eBio5G3 (5G3)), PE, eBioscience™ (Product # 12-1719-42, blue histogram). HeLa Cas9 control cells were also stained ... View More
Product Details
12-1719-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans,
Human
Host/Isotype
Mouse
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio5G3 (5G3)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody eBio5G3 recognizes CD171 also known as neural cell adhesion molecule L1. CD171 is a member of the Ig superfamily containing 6 extracellular Ig domains and five fibronectin type III-like repeats. CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration. Expression of CD171 has been found on monocytes and mature monocytic-derived and follicular DCs, a minor subset of lymphocytes in addition to that found on neuronal tissue and some tumor cells lines. Expression of CD171 on tumors is thought to contribute to tumor progression. Epitope of eBio5G3 is in amino-terminal Ig-like domain.
Applications Reported: This eBio5G3 (5G3) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio5G3 (5G3) antibody has been pre-titrated and tested by flow cytometric analysis of tumor cell line Panc-1. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM
Gene Aliases: CAML1; CD171; HSAS; HSAS1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
UniProt ID: (Human) P32004
Entrez Gene ID: (Human) 3897
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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