|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.125 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The RP/14 monoclonal antibody reacts with mouse CD180 (RP105). This 105 kDa type I transmembrane molecule is a member of the TLR family of proteins characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. RP105 physically associates with another molecule called MD-1 and is expressed on mature B cells, monocytes/macrophages and dendritic cells. Ligation of RP105 with RP/14 protects B cells from irradiation- or dexamethasone-induced apoptosis, and drives them to proliferate. B cells activated by RP105-ligation arrest their growth and undergo apoptosis when the antigen receptor is engaged. The RP105/MD-1 complex in concert with TLR4 mediates B cell recognition and signaling of LPS. Thus, RP105 is a signal transduction molecule and plays a role in regulation of B cell growth and death.
Applications Reported: The RP/14 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The RP/14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells.||Ogata H,Su I,Miyake K,Nagai Y,Akashi S,Mecklenbräuker I,Rajewsky K,Kimoto M,Tarakhovsky A||The Journal of experimental medicine (192:23)||2000|
|Not Applicable||Not Cited||Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression.||Miyake K,Shimazu R,Kondo J,Niki T,Akashi S,Ogata H,Yamashita Y,Miura Y,Kimoto M||Journal of immunology (Baltimore, Md. : 1950) (161:1348)||1998|
|Not Applicable||Not Cited||RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family.||Miyake K,Yamashita Y,Ogata M,Sudo T,Kimoto M||Journal of immunology (Baltimore, Md. : 1950) (154:3333)||1995|