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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD19 Antibodies

          Invitrogen

          CD19 Monoclonal Antibody (eBio1D3 (1D3)), FITC, eBioscience™

          64 Published Figures
          122 References
          View all (353) CD19 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD19 Monoclonal Antibody (eBio1D3 (1D3)), FITC, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (64)
          CD19 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD19 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 65

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          CD19 Antibody (11-0193-82) in Flow

          Staining of BALB/c splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) and 0.125 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.125 µg of Anti-Mouse CD19 FITC (right). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Flow Cytometry (Flow)
          CD19 Antibody in Immunohistochemistry (IHC)

          Product Details

          11-0193-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          0.25 µg/test
          View 119 publications 119 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          eBio1D3 (1D3)

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • Alexa Fluor 561
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • eFluor 506
          • eFluor 660
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 585
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Blue 690
          • NovaFluor Blue 725
          • NovaFluor Blue 760
          • NovaFluor Blue 800
          • NovaFluor Red 710
          • NovaFluor UV 765
          • NovaFluor Violet 690
          • NovaFluor Violet 800
          • NovaFluor Yellow 570
          • NovaFluor Yellow 590
          • NovaFluor Yellow 610
          • NovaFluor Yellow 660
          • NovaFluor Yellow 690
          • NovaFluor Yellow 700
          • NovaFluor Yellow 730
          • NovaFluor Yellow 755
          • NovaFluor Yellow 810
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_657666

          Product Specific Information

          Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

          Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: B-lymphocyte antigen CD19; CD19; Differentiation antigen CD19; Leu-12

          View more View less

          Gene Aliases: AW495831; Cd19

          View more View less

          UniProt ID: (Mouse) P25918

          View more View less

          Entrez Gene ID: (Mouse) 12478

          View more View less

          Process(es)
          chromosome breakage B cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol
          It has to be done as per old AB suggested Products section.
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