Invitrogen

CD19 Monoclonal Antibody (eBio1D3 (1D3)), FITC, eBioscience™

Product Image Gallery

CD19 Antibody — Figure 3. Reduced frequencies of T cell progenitors in Fermt3 -/- thymi. ( A ) Thymocytes from Fermt3 +/+ and Fermt3 -/- mice were stained for lineage markers (B220, CD19, TER119, NK1.1, CD11b, Gr-1, CD8alpha, CD3e, TCRbeta, TCRgammadelta and CD11c), CD44 and c-kit to identify DN 1-2 (Lin neg , c-kit hi , CD44 hi ) and the DN 3-4 (Lin neg , c-kit low , CD44 low ) populations. ( B and C ) PB from Fermt3 +/+ and Fermt3 -/- animals ( P3 ) were stained for lineage markers (TER119, B220, CD11b, Gr-1, CD11c, NK1.1, CD4 and CD8alpha), c-kit and Sca-1 to identify hematopoietic progenitor cells and analysed by flow cytometry. N = 4. Numbers within the representative FACS plots indicate cell percentages. Bars indicate means +- standard errors. **p < 0.01; ***p < 0.001. See also Figure 3--figure supplement 1 . Figure 3--figure supplement 1. Reduced frequencies of T cell prognitors in Fermt3 -/- thymi. Thymocytes from Fermt3 +/+ and Fermt3 -/- P3 mice were stained for lineage markers (B220, CD19, TER119, NK1.1, CD11b, Gr-1, CD8alpha, CD3epsilon, TCRbeta, TCRgammadelta and CD11c), CD44 and CD25 to identify and quantify DN1 (Lin neg , CD44 hi , CD25 - ), DN2 (Lin neg , CD44 hi , CD25 + ), DN3 (Lin neg , CD44 low , CD25 + ) and DN4 (Lin neg , CD44 low , CD25 - ) populations by flow cytometry. Numbers within FACS plots indicate cell percentages. Total number of DN1, DN2, DN3 and DN4 cells were measured by flow cytometry. N = 20. Bars indicate means +- stand

CD19 Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) and 0.125 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.125 µg of Anti-Mouse CD19 FITC (right). Cells in the lymphocyte gate were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.25 µg/test

Published Applications

Flow Cytometry (Flow)

See 62 publications below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1D3 (1D3)

Conjugate

FITC

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_657666

Product Specific Information

Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.