Log fluorescence intensity profiles of human PBLs gated on lymphocytes and analyzed on a BD LSR II flow cytometer (BD Biosciences, San Jose, CA) using 405 nm excitation and the specified emission filters. The data files were analyzed using FlowJo™ software (Treestar, Inc., www.flowjo.com). The black line represents cells stained with anti-human CD2 antibody conjugate and the gray line represents unstained cells. Note: Flow cytometric data shown may not necessarily have been generated using the enclosed lot of reagent. For this reason, and due to differences in flow cytometers and cytometer settings, results may vary from those illustrated above. We recommend titrating reagents to determine optimal conditions for use in your systems.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.
Q10172 was used in flow cytometry to study lymph node-resident simian immunodeficiency virus env-specific T follicular helper cells due to vaccine induction in Rhesus Macaques
|Vargas-Inchaustegui DA,Demers A,Shaw JM,Kang G,Ball D,Tuero I,Musich T,Mohanram V,Demberg T,Karpova TS,Li Q,Robert-Guroff M||Journal of immunology (Baltimore, Md. : 1950) (196:1700)||2016|