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CD2/CD20 Antibody Cocktail, FITC, PE
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Product Details
0220S
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, IgG2a
Class
Cocktail
Type
Antibody
Immunogen
CD2 was derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with t lymphocytes activated by mixed lymphocyte culture.CD20 was derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.
Conjugate
FITC , PE
FITC , PE
FITC , PE
Form
Liquid
Purification
Protein A/G
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Flow Cytometry PBMC: Add 10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
Flow Cytometry Whole Blood: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Target Information
CD2 is found on the majority of human T and NK cells. CD2 presents on 78+8% normal peripheral lymphocytes and 100% of thymocytes. This antibody detects CD2 antigen CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation. It is expressed in all B cell precursor leukemias.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2CD2 antigen; B-lymphocyte cell-surface antigen B1; CD2 antigen (p50), sheep red blood cell receptor; CD2 surface antigen; CD20 antigen; CD20 receptor; erythrocyte receptor; leukocyte surface antigen Leu-16; LFA-3 receptor; lymphocyte-function antigen-2; membrane-spanning 4-domains, subfamily A, member 1; MGC3969; rosette receptor; surface antigen CD2 precursor; T-cell surface antigen CD2 precursor; T-cell surface antigen T11/Leu-5; unnamed protein product
Gene Aliases: B1; Bp35; CD20; CVID5; FMC7; LEU-16; LFA-2; S7; SRBC; T11
UniProt ID: (Human) P06729, (Human) P11836
Entrez Gene ID: (Human) 914, (Human) 931
store-operated calcium entry
humoral immune response
cell surface receptor signaling pathway
B cell differentiation
B cell proliferation
B cell activation
B cell receptor signaling pathway
protein tetramerization
calcium ion import into cytosol
positive regulation of calcium ion import across plasma membrane
membrane raft polarization
apoptotic process
immune response
cell adhesion
natural killer cell activation
positive regulation of myeloid dendritic cell activation
positive regulation of interferon-gamma production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
heterotypic cell-cell adhesion
T cell activation
natural killer cell mediated cytotoxicity
regulation of T cell differentiation
cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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