Immunohistochemical analysis of a hypertrophic human tonsil germinal center using CD20 Polyclonal Antibody (PA1-37312). Frozen sections were fixed in acetone, blocked with 5% normal goat serum, and stained with PA1-37312 followed by a fluorophore-conjugated goat anti-rabbit secondary antibody (red). Tissues were also stained with anti-CD3 (MA1-21199) followed by a fluorophore-conjugated goat anti-mouse secondary antibody (green) and DAPI nuclear stain (blue). Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Published species reactivity||Dog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human CD20|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:300|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
A suggested positive control is tonsil tissue.
CD20 is a 33kDa leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mucosal imbalance of interleukin-1ß and interleukin-1 receptor antagonist in canine inflammatory bowel disease.
PA1-37312 was used in immunohistochemistry to study the role of a mucosal imbalance of interleukin-1? and interleukin-1 receptor antagonist in canine inflammatory bowel disease
|Maeda S,Ohno K,Nakamura K,Uchida K,Nakashima K,Fukushima K,Tsukamoto A,Goto-Koshino Y,Fujino Y,Tsujimoto H||Veterinary journal (London, England : 1997) (194:66)||2012|