|Flow Cytometry (Flow)||5 µL (0.5 µg)/test|
|Flow Cytometry (Flow)||See 1 publications below|
|Host / Isotype||Mouse / IgG2a, kappa|
|Recommended Isotype Control||Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: This 31B monoclonal antibody reacts with human CD208, which is also known as dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP). CD208 is expressed almost exclusively by mature dendritic cells. Expression of this molecule can be induced in monocytes by stimulation with GM-CSF and IL-4. Moreover, CD208 can be upregulated in CD34+-derived dendritic cells by GM-CSF, SCF, and TNFa. CD208 is also expressed in dendritic cells of non-lymphoid tissues such as skin. While CD208 can be detected on the cell surface, this protein is mainly localized within lysosomes. CD208 plays a key role in the processing and presentation of antigen during the immune response.
The 31B antibody only recognizes intracellular CD208, not the cell surface-expressed protein.
Applications Reported: This 31B antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 31B antibody has been pre-titrated and tested by flow cytometric analysis of in vitro differentiated mature dendritic cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD208; DC LAMP; DC-LAMP; DC-lysosome-associated membrane glycoprotein; DCLAMP; LAMP; LAMP-3; lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403
Gene Aliases: CD208; DC LAMP; DC-LAMP; DCLAMP; LAMP; LAMP-3; LAMP3; TSC403
UniProt ID: (Human) Q9UQV4
Entrez Gene ID: (Human) 27074
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