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Invitrogen
CD244 Polyclonal Antibody
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FIGURE: 1 / 5
CD244 Antibody (PA5-106904) in ICC/IF
Immunofluorescence analysis of Natural killer cell receptor 2B4 was performed using 70% confluent log phase NK-92 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD244 Polyclonal Antibody (Product # PA5-106904) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room t... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106904
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CD244(Accession Q9BZW8), corresponding to amino acid residues I9-S59.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CD244.
Target Information
CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via CD244 is thought to modulate NK-cell cytolytic activity. CD244 interacts with SLAMF2, causing the activation of both SLAMF4- and SLAMF2-expressing cells. Patients with systemic lupus erythmatosus have lower than normal levels of SLAMF4 expressed on their NK cells and monocytes, suggesting that SLAMF4 may play a role in the pathology of this autoimmune disease. Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome. Alternatively spliced transcript variants encoding different isoforms of CD244 have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2B4; 2B4S; alternatively spliced; CD244 molecule, natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; cell surface glycoprotein; immunoglobulin superfamily; h2B4; lymphokine-activated killer cell receptor; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell receptor 2B4; NK cell type I receptor protein 2B4; NKR Nmrk; NKR2B4; non-MHC restricted killing associated; secreted form; signaling lymphocytic activation molecule 4; SLAM family member 4; SLAM family receptor; transmembrane form; transmembrane NK cell receptor 2B4; unnamed protein product
Gene Aliases: 2B4; C9.1; Cd244; F730046O15Rik; Ly90; NAIL; NKR2B4; Nmrk; SLAMF4
UniProt ID: (Human) Q9BZW8, (Mouse) Q07763, (Rat) Q9JLM2
Entrez Gene ID: (Human) 51744, (Mouse) 18106, (Rat) 64025
natural killer cell activation involved in immune response
immune response
signal transduction
natural killer cell activation
positive regulation of interferon-gamma production
positive regulation of interleukin-8 production
positive regulation of inositol phosphate biosynthetic process
positive regulation of granzyme B production
myeloid dendritic cell activation
positive regulation of natural killer cell proliferation
positive regulation of CD8-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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