|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Analyte Specific Reagent
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
MHCD2501 was used in flow cytometry to characterize CD4(+)CD25(+) human T cells.
|Ng WF,Duggan PJ,Ponchel F,Matarese G,Lombardi G,Edwards AD,Isaacs JD,Lechler RI||Blood (98:2736)||2001|
Multiple co-stimulatory signals are required for triggering proliferation of T cells from human secondary lymphoid tissue.
MHCD2501 was used in flow cytometry to compare the immune responses of T cells isolated from human secondary lymphoid organs and peripheral blood.
|Agrawal SG,Marquet J,Plumas J,Rouard H,Delfau-Larue MH,Gaulard P,Boumsell L,Reyes F,Bensussan A,Farcet JP||International immunology (13:441)||2001|
Perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.
MHCD2501 was used in flow cytometry to study perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.
|Gopinath R,Hanna LE,Kumaraswami V,Perumal V,Kavitha V,Vijayasekaran V,Nutman TB||Infection and immunity (68:93)||2000|