Staining of PMA and Ionomycin stimulated normal human peripheral blood cells with Anti-Human CD8a APC (cat. 17-0088) and Mouse IgG1 kappa Isotype Control PE (cat. 12-4714) (left) or Anti-Human CD258 (LIGHT) PE (right). Total viable cells were used for analysis.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 µL (0.25 µg)/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Description: This 7-3 (7) monoclonal antibody reacts with human CD258 (also known as LIGHT), a 29-kDa type II transmembrane protein that is a member of the TNF ligand superfamily related to lymphotoxin-β (LT-β). Expressed on activated T cells, particularly CD8+ T cells, LIGHT plays a role in cell survival, inflammation, and tumor eradication. Furthermore, upon binding its ligand, herpesvirus entry mediator (HVEM), LIGHT induces a costimulatory effect on T cells, enhancing T cell proliferation and cytokine production. LIGHT has also been shown to indirectly interact with the LT-β receptor.
Applications Reported: This 7-3 (7) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 7-3 (7) antibody has been pre-titrated and tested by flow cytometric analysis on PMA- and ionomycin-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||LIGHT expression by mucosal T cells may regulate IFN-gamma expression in the intestine.||Cohavy O,Zhou J,Granger SW,Ware CF,Targan SR||Journal of immunology (Baltimore, Md. : 1950) (173:251)||2004|
|Not Applicable||Not Cited||Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects.||Butcher EC,Weissman IL||Journal of immunological methods (37:97)||1981|