|Flow Cytometry (Flow)||5 µL (0.25 µg)/test|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Recommended Isotype Control||Mouse IgG1 kappa Isotype Control, PE, eBioscience™|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: This 7-3 (7) monoclonal antibody reacts with human CD258 (also known as LIGHT), a 29-kDa type II transmembrane protein that is a member of the TNF ligand superfamily related to lymphotoxin-β (LT-β). Expressed on activated T cells, particularly CD8+ T cells, LIGHT plays a role in cell survival, inflammation, and tumor eradication. Furthermore, upon binding its ligand, herpesvirus entry mediator (HVEM), LIGHT induces a costimulatory effect on T cells, enhancing T cell proliferation and cytokine production. LIGHT has also been shown to indirectly interact with the LT-β receptor.
Applications Reported: This 7-3 (7) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 7-3 (7) antibody has been pre-titrated and tested by flow cytometric analysis on PMA- and ionomycin-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; herpesvirus entry mediator A; herpesvirus entry mediator ligand; HVEM-L; HVEML; ligand for herpesvirus entry mediator; LIGHT; LTg; TNFSF14; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; Tumor necrosis factor ligand superfamily member 14; tumor necrosis factor ligand superfamily, member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT
Gene Aliases: CD258; HVEML; LIGHT; LTg; TNFSF14; UNQ391/PRO726
UniProt ID: (Human) O43557
Entrez Gene ID: (Human) 8740
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