Invitrogen

CD275 (B7-H2) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (42) CD275 (B7-H2) antibodies

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CD275 (B7-H2) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ICOS ligand was performed using Daudi and Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD275 (B7-H2) Polyclonal Antibody (Product # PA5-96572) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization for ICOSLG in Daudi cells and panel e represents Jurkat cells showing no staining for the samel. Panel f represents control Daudi cells with no primary antibody to assess background. The images were captured at 60X with EVOS™ M7000 Imaging System (Product # AMF7000) magnification.

CD275 (B7-H2) Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of CD275 (B7-H2) in paraffin-embedded human skin carcinoma using CD275 (B7-H2) Polyclonal Antibody (Product # PA5-96572) at a dilution of 1:100.

CD275 (B7-H2) Antibody in Western Blot (WB) — Western Blot analysis of CD275 (B7-H2) in extracts of various cell lines using CD275 (B7-H2) Polyclonal Antibody (Product # PA5-96572) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

CD275 (B7-H2) Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD275 (B7-H2) Polyclonal Antibody (Product # PA5-96572) shows membrane staining for ICOSLG in Daudi and not in Jurkat, which is reported low expressing for the protein. {RE}

Product Details

PA5-96572

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50 - 1:200	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 19-256 of human ICOSLG. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.7 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2808374

Product Specific Information

Immunogen sequence: DTQEKEVRAM VGSDVELSCA CPEGSRFDLN DVYVYWQTSE SKTVVTYHIP QNSSLENVDS RYRNRALMSP AGMLRGDFSL RLFNVTPQDE QKFHCLVLSQ SLGFQEVLSV EVTLHVAANF SVPVVSAPHS PSQDELTFTC TSINGYPRPN VYWINKTDNS LLDQALQNDT VFLNMRGLYD VVSVLRIART PSVNIGCCIE NVLLQQNLTV GSQTGNDIGE RDKITENPVS TGEKNAAT; Positive Samples: HL-60, BT-474, Mouse heart, Mouse brain, Rat spleen; Cellular Location: Membrane, Single-pass type I membrane protein

Target Information

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B7 homolog 2; B7 homologue 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275; ICOS ligand; inducible T-cell co-stimulator ligand; LICOS; transmembrane protein B7-H2 ICOS ligand

Gene Aliases: AU044799; B7-H2; B7h; B7H2; B7RP-1; B7RP1; BG071784; CD275; GI50; GL50; GL50-B; ICOS-L; ICOSL; ICOSLG; KIAA0653; LICOS; Ly115l; mKIAA0653; RGD1562791

UniProt ID: (Human) O75144, (Mouse) Q9JHJ8

Entrez Gene ID: (Human) 23308, (Mouse) 50723, (Rat) 499415

Molecular Function: immunoglobulin receptor superfamily