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Search Thermo Fisher Scientific
Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (IHC) |
Assay-Dependent | - |
Flow Cytometry (Flow) |
0.25 µg/test | View 15 publications 15 publications |
Immunoprecipitation (IP) |
Assay-Dependent | - |
Functional Assay (FN) |
Assay-Dependent | View 2 publications 2 publications |
Blocking Assay (BLOCK) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse, Rhesus monkey, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Armenian hamster / IgG |
Class |
Monoclonal |
Type |
Antibody |
Clone |
C398.4A |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
RRID |
AB_468637 |
Description: The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e., C398.4A does not block binding of ICOSL).
Applications Reported: The C398.4A antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. This has also been reported for in vitro functional assays. This mAb does not work for immunoblotting (WB). (Please use Functional Grade purified C398.4A, cat. 16-9949, in functional assays.).
Applications Tested: This C398.4A antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator
Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115
UniProt ID: (Rat) Q9R1T7, (Mouse) Q9WVS0
Entrez Gene ID: (Rhesus monkey) 705788, (Rat) 64545, (Mouse) 54167
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