Invitrogen

CD278 (ICOS) Monoclonal Antibody (C398.4A), eBioscience™

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CD278 (ICOS) Antibody in Flow Cytometry (Flow) — Staining of Con A-stimulated mouse splenocytes with 0.25 µg of Anti-Mouse/Rat CD278 (ICOS) Purified (filled histogram) or 0.25 µg of Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111).Total viable cells were used for analysis.

Product Details

14-9949-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	Assay-Dependent	-
Flow Cytometry (Flow)	0.25 µg/test	View 15 publications 15 publications
Immunoprecipitation (IP)	Assay-Dependent	-
Functional Assay (FN)	Assay-Dependent	View 2 publications 2 publications
Blocking Assay (BLOCK)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse, Rhesus monkey, Rat
Published species	Human, Mouse
Host/Isotype	Armenian hamster / IgG
Class	Monoclonal
Type	Antibody
Clone	C398.4A
Conjugate	Unconjugated APC APC-eFluor 780 Biotin FITC Functional Grade PE PE-Cyanine5 PE-Cyanine7 Super Bright 436
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_468637

Product Specific Information

Description: The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e., C398.4A does not block binding of ICOSL).

Applications Reported: The C398.4A antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. This has also been reported for in vitro functional assays. This mAb does not work for immunoblotting (WB). (Please use Functional Grade purified C398.4A, cat. 16-9949, in functional assays.).

Applications Tested: This C398.4A antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

T-B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein.

Journal of immunology (Baltimore, Md. : 1950)

Bonami RH,Nyhoff LE,McNitt DH,Hulbert C,Felton JL,Kendall PL,Thomas JW

14-9949-82 was used in Flow Cytometry to understand how fully developed T follicular helper cells contribute to different autoimmune processes, investigating the role of signaling lymphocytic activation molecule-associated protein in type 1 diabetes and autoantibody-mediated arthritis.

Tue Dec 15 00:00:00 EST 2020

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9949-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

The Kinase Complex mTOR Complex 2 Promotes the Follicular Migration and Functional Maturation of Differentiated Follicular Helper CD4⁺ T Cells During Viral Infection.

Frontiers in immunology

Hao Y,Wang Y,Liu X,Yang X,Wang P,Tian Q,Bai Q,Chen X,Li Z,Wu J,Xie Z,Zhou X,Zhou Y,Yin Z,Wu Y,Ye L

Tue Jul 30 00:00:00 EDT 2019

The E3 ligase VHL controls alveolar macrophage function via metabolic-epigenetic regulation.

The Journal of experimental medicine

Zhang W,Li Q,Li D,Li J,Aki D,Liu YC

14-9949 was used in Flow cytometry/Cell sorting to show VHL regulates alveolar macrophages and acts as a crucial egulatory factor in lung inflammation and fibrosis.

Mon Dec 03 00:00:00 EST 2018

Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b.

Nature communications

Cho S,Lee HM,Yu IS,Choi YS,Huang HY,Hashemifar SS,Lin LL,Chen MC,Afanasiev ND,Khan AA,Lin SW,Rudensky AY,Crotty S,Lu LF

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9949-82) in Flow Cytometry

Mon Jul 16 00:00:00 EDT 2018

The transcription factor Runx3 guards cytotoxic CD8⁺ effector T cells against deviation towards follicular helper T cell lineage.

Nature immunology

Shan Q,Zeng Z,Xing S,Li F,Hartwig SM,Gullicksrud JA,Kurup SP,Van Braeckel-Budimir N,Su Y,Martin MD,Varga SM,Taniuchi I,Harty JT,Peng W,Badovinac VP,Xue HH

14-9949 was used in Flow cytometry/Cell sorting to show that Runx3-mediated Tcf7 repression coordinately enforced acquisition of cytotoxic functions and protected the cytotoxic lineage integrity by preventing TFH-lineage deviation.

Tue Aug 01 00:00:00 EDT 2017

Characterization of c-Maf⁺Foxp3^- Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells.

Scientific reports

Chien CH,Yu HC,Chen SY,Chiang BL

14994982 was used in flow cytometry to generate and characterize Treg-of-B cells

Wed Apr 12 00:00:00 EDT 2017

Tumor Progression Locus 2 (Tpl2) Activates the Mammalian Target of Rapamycin (mTOR) Pathway, Inhibits Forkhead Box P3 (FoxP3) Expression, and Limits Regulatory T Cell (Treg) Immunosuppressive Functions.

The Journal of biological chemistry

Li X,Acuff NV,Peeks AR,Kirkland R,Wyatt KD,Nagy T,Watford WT

14-9949-82 was used in Flow Cytometry to demonstrate that the serine/threonine kinase tumour progression locus 2 constrains the development of inducible regulatory T cells.

Fri Aug 05 00:00:00 EDT 2016

LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.

Nature immunology

Choi YS,Gullicksrud JA,Xing S,Zeng Z,Shan Q,Li F,Love PE,Peng W,Xue HH,Crotty S

14-9949 was used in Flow cytometry/Cell sorting to report that selective loss of Lef1 or Tcf7 results in T(FH) cell defects.

Tue Sep 01 00:00:00 EDT 2015

Dendritic Cell-Based Vaccines that Utilize Myeloid Rather than Plasmacytoid Cells Offer a Superior Survival Advantage in Malignant Glioma.

Journal of immunology (Baltimore, Md. : 1950)

Dey M,Chang AL,Miska J,Wainwright DA,Ahmed AU,Balyasnikova IV,Pytel P,Han Y,Tobias A,Zhang L,Qiao J,Lesniak MS

14-9949 was used in Flow cytometry/Cell sorting to compare the ability of myeloid and plasmacytoid dendritic cells to generate effective antiglioma immunity.

Wed Jul 01 00:00:00 EDT 2015

ICOS coreceptor signaling inactivates the transcription factor FOXO1 to promote Tfh cell differentiation.

Immunity

Stone EL,Pepper M,Katayama CD,Kerdiles YM,Lai CY,Emslie E,Lin YC,Yang E,Goldrath AW,Li MO,Cantrell DA,Hedrick SM

14994982 was used in blocking or activating experiment to explore the relationship between ICOS signaling and FOXO1 in T follicular helper cells

Tue Feb 17 00:00:00 EST 2015

ICOS coreceptor signaling inactivates the transcription factor FOXO1 to promote Tfh cell differentiation.

Immunity

Stone EL,Pepper M,Katayama CD,Kerdiles YM,Lai CY,Emslie E,Lin YC,Yang E,Goldrath AW,Li MO,Cantrell DA,Hedrick SM

14994982 was used in blocking or activating experiment to explore the relationship between ICOS signaling and FOXO1 in T follicular helper cells

Tue Feb 17 00:00:00 EST 2015

Modulation of distinct asthmatic phenotypes in mice by dose-dependent inhalation of microbial products.

Environmental health perspectives

Whitehead GS,Thomas SY,Cook DN

14-9949 was used in Flow cytometry/Cell sorting to assess pulmonary cytokine production and airway inflammation following challenge with ovalbumin and LPS.

Wed Jan 01 00:00:00 EST 2014

Phosphatidylinositol 3-kinase-independent signaling pathways contribute to ICOS-mediated T cell costimulation in acute graft-versus-host disease in mice.

Journal of immunology (Baltimore, Md. : 1950)

Li J,Heinrichs J,Leconte J,Haarberg K,Semple K,Liu C,Gigoux M,Kornete M,Piccirillo CA,Suh WK,Yu XZ

14-9949 was used in Functional assays to investigate ICOS signal transduction pathways involved in graft-versus-host disease, showing that PI3K--independent signaling pathways contribute to ICOS-mediated T cell costimulation.

Mon Jul 01 00:00:00 EDT 2013

Augmented ICOS expression in patients with early diffuse cutaneous systemic sclerosis.

Rheumatology (Oxford, England)

Hasegawa M,Fujimoto M,Matsushita T,Hamaguchi Y,Takehara K

14-9949 was used in Functional assays to determine the contribution of ICOS signalling to the pathogenesis of systemic sclerosis during early progressive disease.

Fri Feb 01 00:00:00 EST 2013

The ICOS/ICOSL pathway is required for optimal antitumor responses mediated by anti-CTLA-4 therapy.

Cancer research

Fu T,He Q,Sharma P

14-9949 was used in Flow cytometry/Cell sorting to identify mechanisms that elicit clinical benefit in the setting of anti-CTLA-4 therapy.

Mon Aug 15 00:00:00 EDT 2011

Foxp3+ regulatory T cells control humoral autoimmunity by suppressing the development of long-lived plasma cells.

Journal of immunology (Baltimore, Md. : 1950)

Jang E,Cho WS,Cho ML,Park HJ,Oh HJ,Kang SM,Paik DJ,Youn J

14-9949 was used in Flow cytometry/Cell sorting to reveal that Tregs suppress the emergence of long-lived splenic plasma cells by affecting plasma cell-autonomous mechanisms.

Tue Feb 01 00:00:00 EST 2011

ICOS controls effector function but not trafficking receptor expression of kidney-infiltrating effector T cells in murine lupus.

Journal of immunology (Baltimore, Md. : 1950)

Odegard JM,DiPlacido LD,Greenwald L,Kashgarian M,Kono DH,Dong C,Flavell RA,Craft J

14-9949 was used in Flow cytometry/Cell sorting to show that ICOS is strictly required for local inflammatory functions of autoreactive CD4 T cells in the renal pathology of murine lupus.

Wed Apr 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

UniProt ID: (Rat) Q9R1T7, (Mouse) Q9WVS0

Entrez Gene ID: (Rhesus monkey) 705788, (Rat) 64545, (Mouse) 54167

Function(s)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Process(es)

molecular_function protein binding

CD278 (ICOS) Monoclonal Antibody (C398.4A), eBioscience™

Product Details

14-9949-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (FN)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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