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Invitrogen
CD33 Polyclonal Antibody
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FIGURE: 1 / 2
CD33 Antibody (PA5-97561) in IHC (P)
Immunohistochemical analysis of CD33 in paraffin embedded human placenta tissue using a CD33 polyclonal antibody (Product # PA5-97561) at a dilution of 1:300. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detec... View More
Product Details
PA5-97561
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Myeloid cell surface antigen CD33 protein (18-259AA)
Conjugate
Form
Liquid
Concentration
4.41 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD33; CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; lacks exon 2 but includes exon 7a; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; unnamed protein product
Gene Aliases: CD33; CD33rSiglec; p67; SIGLEC-3; SIGLEC3
UniProt ID: (Human) P20138
Entrez Gene ID: (Human) 945
immune response-inhibiting signal transduction
cell adhesion
signal transduction
cell-cell signaling
negative regulation of cell proliferation
negative regulation of interleukin-1 beta production
negative regulation of interleukin-8 production
negative regulation of tumor necrosis factor production
Fc-gamma receptor signaling pathway
positive regulation of protein secretion
cell-cell adhesion
negative regulation of monocyte activation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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