|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 7 publications below|
MA5-17444 targets CD4 in FACS applications and shows reactivity with Mouse samples.
The MA5-17444 immunogen is mouse thymocytes.
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IL-18 enhances IL-4 production by ligand-activated NKT lymphocytes: a pro-Th2 effect of IL-18 exerted through NKT cells.
MA5-17444 was used in flow cytometry to investigate the effect of IL-18 on NKT cells.
|Leite-De-Moraes MC,Hameg A,Pacilio M,Koezuka Y,Taniguchi M,Van Kaer L,Schneider E,Dy M,Herbelin A||Journal of immunology (Baltimore, Md. : 1950) (166:945)||2001|
Genetic regulation of commitment to interleukin 4 production by a CD4(+) T cell-intrinsic mechanism.
MA5-17444 was used in flow cytometry to use T cells from BALB mice and study IL-4 production.
|Bix M,Wang ZE,Thiel B,Schork NJ,Locksley RM||The Journal of experimental medicine (188:2289)||1998|
Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.
MA5-17444 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.
|Liu L,Rich BE,Inobe J,Chen W,Weiner HL||International immunology (10:1017)||1998|
Surface antigen expression in spleen cells of C57B1/6 mice during ageing: influence of sex and parity.
MA5-17444 was used in flow cytometry to test if pregnancies and gender influence the cellar changes observed during ageing in mice.
|Barrat F,Lesourd BM,Louise A,Boulouis HJ,Vincent-Naulleau S,Thibault D,Sanaa M,Neway T,Pilet CH||Clinical and experimental immunology (107:593)||1997|
Exclusive development of T cell neoplasms in mice transplanted with bone marrow expressing activated Notch alleles.
MA5-17444 was used in flow cytometry to report that TAN1 is an oncoprotein.
|Pear WS,Aster JC,Scott ML,Hasserjian RP,Soffer B,Sklar J,Baltimore D||The Journal of experimental medicine (183:2283)||1996|
Pathogenic and protective roles of CD45RB(low) CD4+ cells correlate with cytokine profiles in the spontaneously autoimmune diabetic mouse.
MA5-17444 was used in flow cytometry to test if CD45RB(low) population of CD4+ cells is protective or pathogenic to the development of disease in the NOD mouse.
|Shimada A,Rohane P,Fathman CG,Charlton B||Diabetes (45:71)||1996|
Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation.
MA5-17444 was used in flow cytometry to investigate KMI6 recognition of beta 1 integrins on T cells.
|Wadsworth SA,Chang AC,Hong MJ,Halvorson MJ,Otto S,Coligan JE||Journal of immunology (Baltimore, Md. : 1950) (154:2125)||1995|