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CD4/CD28 Antibody Cocktail, FITC, PE

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CD4/CD28 Antibody Cocktail, FITC, PE

Product Details

0428S

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

10 µL/1x10^6 cells in a 100 µL suspension
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Immunogen

CD4 was derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD28 was derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
3D Epitope / Immunogen

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Flow Cytometry PBMC: Add 10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

Flow Cytometry Whole Blood: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Target Information

The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD28; CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; MGC138290; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell-specific homodimer surface protein precursor; T-cell-specific surface glycoprotein CD28; T4 surface glycoprotein precursor; TP44; unnamed protein product

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Gene Aliases: CD28; CD4; CD4mut; IMD123; IMD79; Leu-3; OKT4D; T4; Tp44

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UniProt ID: (Human) P10747, (Human) P01730

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Entrez Gene ID: (Human) 940, (Human) 920

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Function(s)
protein binding coreceptor activity protein kinase binding identical protein binding virus receptor activity transmembrane signaling receptor activity receptor binding extracellular matrix structural constituent zinc ion binding lipid binding immunoglobulin binding enzyme binding MHC class II protein complex binding signaling receptor activity interleukin-16 binding interleukin-16 receptor activity MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding
Process(es)
positive regulation of protein phosphorylation adaptive immune response immune system process positive regulation of immune system process immune response cell adhesion signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol calcium-mediated signaling T cell differentiation macrophage differentiation response to estradiol maintenance of protein location in cell positive regulation of interleukin-2 production response to vitamin D positive regulation of kinase activity helper T cell enhancement of adaptive immune response interleukin-15-mediated signaling pathway positive regulation of T cell proliferation T cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade T cell selection response to ethanol positive regulation of monocyte differentiation positive regulation of protein kinase activity positive regulation of transcription, DNA-templated positive regulation of viral entry into host cell viral entry into host cell defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation positive regulation of multicellular organismal process regulation of calcium ion transport positive regulation of ERK1 and ERK2 cascade cellular response to granulocyte macrophage colony-stimulating factor stimulus response to methamphetamine hydrochloride cellular response to ionomycin positive regulation of cytokine production positive regulation of inflammatory response to antigenic stimulus transcription from RNA polymerase II promoter humoral immune response positive regulation of gene expression negative regulation of gene expression T cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-4 production CD4-positive, alpha-beta T cell proliferation T cell proliferation regulation of T cell proliferation negative regulation of apoptotic process protein kinase B signaling negative thymic T cell selection regulatory T cell differentiation positive regulation of viral genome replication regulation of regulatory T cell differentiation positive regulation of translation positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgG isotypes T cell receptor signaling pathway positive regulation of protein kinase B signaling apoptotic signaling pathway positive regulation of CD4-positive, alpha-beta T cell proliferation
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