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CD4/CD8 Antibody Cocktail, FITC, PE

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CD4/CD8 Antibody Cocktail, FITC, PE

Product Details

0048S

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

10 µL/1x10^6 cells in a 100 µL suspension
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Immunogen

CD4 was derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD8 was derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
3D Epitope / Immunogen

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS with 0.2% carrier protein

Contains

0.08% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Flow Cytometry PBMC: Add 10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

Flow Cytometry Whole Blood: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Target Information

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8a molecule; CD8alpha; CD8b; CD8b molecule; CD8beta; cell surface glycoprotein T8 precursor; Leu-2; Leu2 T-lymphocyte antigen; Ly-3 homolog; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein (CD8-beta) precursor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell differentiation antigen Leu-2/T8; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T4 surface glycoprotein precursor; T8 T-cell antigen; unnamed protein product

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Gene Aliases: CD4; CD4mut; CD8; CD8A; CD8alpha; CD8B; CD8B1; CD8beta; IMD116; IMD79; Leu-3; LEU2; Ly-3; LY3; LYT3; MAL; OKT4D; p32; P37; T4

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UniProt ID: (Human) P01730, (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 920, (Human) 925, (Human) 926

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Function(s)
virus receptor activity transmembrane signaling receptor activity receptor binding extracellular matrix structural constituent protein binding zinc ion binding lipid binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein complex binding signaling receptor activity interleukin-16 binding interleukin-16 receptor activity MHC class II protein binding identical protein binding protein homodimerization activity protein tyrosine kinase binding MHC class I protein complex binding MHC class I protein binding
Process(es)
adaptive immune response immune system process immune response transmembrane receptor protein tyrosine kinase signaling pathway T cell activation regulation of immune response T cell receptor signaling pathway positive regulation of protein phosphorylation positive regulation of immune system process cell adhesion signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway positive regulation of calcium ion transport into cytosol calcium-mediated signaling T cell differentiation macrophage differentiation response to estradiol maintenance of protein location in cell positive regulation of interleukin-2 production response to vitamin D positive regulation of kinase activity helper T cell enhancement of adaptive immune response interleukin-15-mediated signaling pathway positive regulation of T cell proliferation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade T cell selection response to ethanol positive regulation of monocyte differentiation positive regulation of protein kinase activity positive regulation of transcription, DNA-templated positive regulation of viral entry into host cell viral entry into host cell defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation positive regulation of multicellular organismal process regulation of calcium ion transport positive regulation of ERK1 and ERK2 cascade cellular response to granulocyte macrophage colony-stimulating factor stimulus response to methamphetamine hydrochloride cellular response to ionomycin T cell mediated immunity antigen processing and presentation cytotoxic T cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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