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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Analyte Specific Reagent
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
MHCD4515 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.
|Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M||Mucosal immunology (9:159)||2016|
Integrated nanoplasmonic sensing for cellular functional immunoanalysis using human blood.
MHCD4515 was used in flow cytometry to use localized surface plasmon resonance biosensors to cytokine production by human blood.
|Oh BR,Huang NT,Chen W,Seo JH,Chen P,Cornell TT,Shanley TP,Fu J,Kurabayashi K||ACS nano (8:2667)||2014|
EphB6 crosslinking results in costimulation of T cells.
MHCD4515 was used in flow cytometry to report that EphB6 and its ligands facilitate T cell responses to antigen.
|Luo H,Yu G,Wu Y,Wu J||The Journal of clinical investigation (110:1141)||2002|
B220; c polypeptide; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; Leukocyte common antigen; LY5; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; receptor type; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200