Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?
Frontiers in immunology
Ehlers AM,den Hartog Jager CF,Kardol-Hoefnagel T,Katsburg MMD,Knulst AC,Otten HG
MHCD4530 was used in Flow cytometry/Cell sorting to compare strategies to generate human mAbs from peripheral blood regarding costs, time expenditure, recovery, throughput and complexity.
Mon Oct 18 00:00:00 EDT 2021
Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity.
Szodoray P,Andersen TK,Heinzelbecker J,Imbery JF,Huszthy PC,Stanford SM,Bogen B,Landsverk OB,Bottini N,Tveita A,Munthe LA,Nakken B
MHCD4530 was used in Flow Cytometry to show that high CD45 phosphatase activity in memory B cells enhances B cell receptor signaling and is essential for their effective antibody-secreting cell differentiation.
Tue Aug 10 00:00:00 EDT 2021
Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.
Du W,Hua F,Li X,Zhang J,Li S,Wang W,Zhou J,Wang W,Liao P,Yan Y,Li G,Wei S,Grove S,Vatan L,Zgodziński W,Majewski M,Wallner G,Chen H,Kryczek I,Fang JY,Zou W
MHCD4530 was used in Flow Cytometry to show that optineurin is a shared node between the IFN and MHC pathways and predicts colorectal cancer patient outcome.
Thu Jul 01 00:00:00 EDT 2021
Maturation-associated gene expression profiles during normal human bone marrow erythropoiesis.
Cell death discovery
Mello FV,Land MGP,Costa ES,Teodósio C,Sanchez ML,Bárcena P,Peres RT,Pedreira CE,Alves LR,Orfao A
MHCD4530 was used in Flow Cytometry to investigate the ex vivo gene expression profile of normal bone marrow human erythroid precursor cells at different maturation stages.
Thu Oct 01 00:00:00 EDT 2020
Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples.
Journal of immunological methods
van der Velden VHJ,Flores-Montero J,Perez-Andres M,Martin-Ayuso M,Crespo O,Blanco E,Kalina T,Philippé J,Bonroy C,de Bie M,Te Marvelde J,Teodosio C,Corral Mateos A,Kanderová V,van der Burg M,Van Hoof D,van Dongen JJM,Orfao A
MHCD4530 was used in flow cytometry to assess the use of dried formats of the lymphocytosis screening tube and of the primary immune deficiency orientation tube
Sun Dec 01 00:00:00 EST 2019
Scalable high-throughput acoustophoresis in arrayed plastic microchannels.
Dubay R,Lissandrello C,Swierk P,Moore N,Doty D,Fiering J
MHCD4530TR was used in Flow cytometry/Cell sorting to suggest that acoustophoresis could in the future aid in cell therapy bioprocessing and that further scale-up is possible.
Wed May 01 00:00:00 EDT 2019
Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture system.
Ickrath P,Scherzad A,Kleinsasser N,Ginzkey C,Hagen R,Hackenberg S
MHCD4530 was used in Flow cytometry/Cell sorting to assess the modulatory influence of the inflamed nasal polyp epithelial cells on the phenotype of T cells.
Fri Feb 01 00:00:00 EST 2019
Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis.
International journal of molecular medicine
Ickrath P,Kleinsasser N,Ding X,Ginzkey C,Beyersdorf N,Hagen R,Kerkau T,Hackenberg S
MHCD4530 was used in Flow cytometry/Cell sorting to characterise CD69+ sphingosine-1-phosphate receptor 1 tissue resident memory T cells in the polyps of patients with chronic rhinosinusitis with nasal polyps.
Wed Aug 01 00:00:00 EDT 2018
Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.
Mieczkowska A,Schumacher A,Filipowicz N,Wardowska A,Zieliński M,Madanecki P,Nowicka E,Langa P,Deptuła M,Zieliński J,Kondej K,Renkielska A,Buckley PG,Crossman DK,Crowley MR,Czupryn A,Mucha P,Sachadyn P,Janus Ł,Skowron P,Rodziewicz-Motowidło S,Cichorek M,Pikuła M,Piotrowski A
MHCD4530 was used in Flow cytometry/Cell sorting to study the impact of prolonged cell culture on the adipose-derived stem cell phenotype and their differentiation potential.
Fri Jul 27 00:00:00 EDT 2018
Targeting of colony-stimulating factor 1 receptor (CSF1R) in the CLL microenvironment yields antineoplastic activity in primary patient samples.
Edwards V DK,Sweeney DT,Ho H,Eide CA,Rofelty A,Agarwal A,Liu SQ,Danilov AV,Lee P,Chantry D,McWeeney SK,Druker BJ,Tyner JW,Spurgeon SE,Loriaux MM
MHCD4530 was used in Flow cytometry/Cell sorting to demonstrate the preclinical activity of two inhibitors of CSF1R, GW-2580 and ARRY-382, in primary chronic lymphocytic leukaemia patient samples.
Tue May 15 00:00:00 EDT 2018
Immunodeficiency in Bloom's Syndrome.
Journal of clinical immunology
Schoenaker MHD,Henriet SS,Zonderland J,van Deuren M,Pan-Hammarström Q,Posthumus-van Sluijs SJ,Pico-Knijnenburg I,Weemaes CMR,IJspeert H
MHCD4530 was used in Flow cytometry/Cell sorting to perform immune phenotyping of the B and T cells and analyzed the SHM and CSR in detail by analyzing IGHA and IGHG transcripts using next-generation sequencing.
Mon Jan 01 00:00:00 EST 2018
Genetic defects in PI3Kδ affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections.
Clinical immunology (Orlando, Fla.)
Wentink M,Dalm V,Lankester AC,van Schouwenburg PA,Schölvinck L,Kalina T,Zachova R,Sediva A,Lambeck A,Pico-Knijnenburg I,van Dongen JJ,Pac M,Bernatowska E,van Hagen M,Driessen G,van der Burg M
MHCD4530 was used in flow cytometry to discuss how mutations in PIK3CD and PIK3R1 cause activated PI3K-delta syndrome
Wed Mar 01 00:00:00 EST 2017
The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children.
The Journal of allergy and clinical immunology
van Erp FC,Knol EF,Pontoppidan B,Meijer Y,van der Ent CK,Knulst AC
MHCD4530 was used in flow cytometry to identify peanut proteins that can predict peanut allergy.
Sun Jan 01 00:00:00 EST 2017
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M
MHCD4530 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.
Fri Jan 01 00:00:00 EST 2016
Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα.
Journal of leukocyte biology
Chao Y,Kaliaperumal N,Chretien AS,Tang S,Lee B,Poidinger M,Fairhurst AM,Connolly JE
MHCD4530 was used in Flow cytometry/Cell sorting to explore the role of IL-18R signalling in IFN-α production in human plasmacytoid dendritic cells.
Mon Dec 01 00:00:00 EST 2014
In vitro immunomodulation of a whole blood IFN-γ release assay enhances T cell responses in subjects with latent tuberculosis infection.
Gaur RL,Suhosk MM,Banaei N
MHCD4530 was used in flow cytometry to discuss the use of PPR ligands as adjuvants and the use of interferon-gamma release assays as a diagnostic tool
Tue Aug 26 00:00:00 EDT 2014
Immune response to JC virus T antigen in patients with and without colorectal neoplasia.
Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR
MHCD4530 was used in flow cytometry to study the cell mediated immune response to JCV T-Ag in patients with colorectal adenomatous polyps or cancers
Tue Jul 01 00:00:00 EDT 2014
Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice.
Journal of immunology (Baltimore, Md. : 1950)
Billerbeck E,Horwitz JA,Labitt RN,Donovan BM,Vega K,Budell WC,Koo GC,Rice CM,Ploss A
MHCD4530 was used in flow cytometry to investigate the role of HLA molecules during infection using humanized mice
Thu Aug 15 00:00:00 EDT 2013
FcRL5 as a target of antibody-drug conjugates for the treatment of multiple myeloma.
Molecular cancer therapeutics
Elkins K,Zheng B,Go M,Slaga D,Du C,Scales SJ,Yu SF,McBride J,de Tute R,Rawstron A,Jack AS,Ebens A,Polson AG
MHCD4530 was used in flow cytometry to investigate the use of FcRL5 antibody-drug conjugates as a treatment for multiple myeloma.
Mon Oct 01 00:00:00 EDT 2012
Rapid ex vivo isolation and long-term culture of human Th17 cells.
Journal of immunological methods
Streeck H,Cohen KW,Jolin JS,Brockman MA,Meier A,Power KA,Waring MT,Alter G,Altfeld M
MHCD4530 was used in flow cytometry to describe a novel technique to isolate viable Th17 cells based on their ability to secrete IL-17.
Sun Apr 20 00:00:00 EDT 2008
Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.
European heart journal
Leone AM,Rutella S,Bonanno G,Abbate A,Rebuzzi AG,Giovannini S,Lombardi M,Galiuto L,Liuzzo G,Andreotti F,Lanza GA,Contemi AM,Leone G,Crea F
MHCD4530 was used in Flow cytometry/Cell sorting to assess the spontaneous mobilisation of bone marrow-derived stem cells expressing CD34 following acute myocardial infarction, and its relation to post-infarction remodelling.
Wed Jun 01 00:00:00 EDT 2005
Analysis of CD10+ hairy cell leukemia.
American journal of clinical pathology
Jasionowski TM,Hartung L,Greenwood JH,Perkins SL,Bahler DW
MHCD4530 was used in flow cytometry to examine CD10 expression in hairy cell leukemia.
Fri Aug 01 00:00:00 EDT 2003
Prospective isolation of human clonogenic common myeloid progenitors.
Proceedings of the National Academy of Sciences of the United States of America
Manz MG,Miyamoto T,Akashi K,Weissman IL
MHCD4530 was used in flow cytometry to report how to isolate highly purified hematopoietic intermediates.
Tue Sep 03 00:00:00 EDT 2002
Precise CD4 T-cell counting using red diode laser excitation: for richer, for poorer.
Janossy G,Jani IV,Kahan M,Barnett D,Mandy F,Shapiro H
MHCD4530 was used in flow cytometry to discuss the high cost of measuring CD4 T cells by flow cytometry.
Mon Apr 15 00:00:00 EDT 2002
Short-term, serum-free, static culture of cord blood-derived CD34+ cells: effects of FLT3-L and MIP-1alpha on in vitro expansion of hematopoietic progenitor cells.
Capmany G,Querol S,Cancelas JA,García J
MHCD4530 was used in flow cytometry to identify the optimal condition for the generation of committed progenitors from cord blood cells without affecting the stem cell compartment.
Sun Aug 01 00:00:00 EDT 1999
Illegitimate transcription: its use for studying genetic abnormalities in lymphoblastoid cells from patients with Glanzmann thrombasthenia.
British journal of haematology
Negrier C,Vinciguerra C,Attali O,Grenier C,Larcher ME,Dechavanne M
MHCD4530 was used in flow cytometry to study RNA transcripts in patients with Glanzmann thrombasthenia.
Thu Jan 01 00:00:00 EST 1998
Growth of human hematopoietic cells in immunodeficient mice conditioned with cyclophosphamide and busulfan.
Stem cells (Dayton, Ohio)
Basch RS,Quito FL,Beh J,Hirst JA
MHCD4530 was used in immunocytochemistry to report that human hematopoietic cells survive and proliferate in severe combined immunodeficient mice treated with busulfan and cyclophosphamide.
Thu Oct 16 00:00:00 EDT 1997