|Tested species reactivity||Bovine, Goat, Sheep|
|Published species reactivity||Bovine, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Ovine efferent lymphatic duct lymphocytes.|
|Storage buffer||PBS, pH 7.4, with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.
MA1-81458 was used in flow cytometry to study how the IL6R/STAT3 signaling pathway regulates IL-8 production by primary endometrial cells in response to lipopolysaccharide treatment
|Cronin JG,Kanamarlapudi V,Thornton CA,Sheldon IM||Mucosal immunology (9:1125)||2016|
Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1, and TLR6.
MA1-81458 was used in flow cytometry to study the role of TLR-1, -2 and -6 on bovine endometrial stromal and epithelial cells in the immune and inflammatory responses to bacterial lipopeptides
|Turner ML,Cronin JG,Healey GD,Sheldon IM||Endocrinology (155:1453)||2014|
The evaluation of leukocytes in response to the in vitro testing of ventricular assist devices.
MA1-81458 was used in flow cytometry to study the effects of ventricular assist pumps on leukocytes using an in vitro test system
|Chan CH,Hilton A,Foster G,Hawkins KM,Badiei N,Thornton CA||Artificial organs (37:793)||2013|