|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||The CD45 antigen|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD11b+ and Sca-1+ Cells Exert the Main Beneficial Effects of Systemically Administered Bone Marrow-Derived Mononuclear Cells in a Murine Model of Mixed Th2/Th17 Allergic Airway Inflammation.
MCD4501 was used in flow cytometry to Sca-1+ and CD11b+ cells exert the main beneficial effects of systemically administered bone marrow-derived mononuclear cells by use of a murine model of mixed Th2/Th17 allergic airway inflammation
|Cruz FF,Borg ZD,Goodwin M,Coffey AL,Wagner DE,Rocco PR,Weiss DJ||Stem cells translational medicine (5:488)||2016|
CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1.
MCD4501 was used in flow cytometry to test if upregulation of major histocompatibility complex class II and costimulatory molecules in the retina contributes to the regulation of CD4 T cells
|Charles E,Joshi S,Ash JD,Fox BA,Farris AD,Bzik DJ,Lang ML,Blader IJ||Infection and immunity (78:3484)||2010|