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Invitrogen

CD45 Monoclonal Antibody (30-F11), PE-Texas Red

4 Published Figures
15 References
View all (457) CD45 antibodies

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Product Details

MCD4517

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-dependent
View 15 publications 15 publications
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

30-F11

Immunogen

The CD45 antigen

Conjugate

PE-Texas Red PE-Texas Red PE-Texas Red

Excitation/Emission Max

567/615 nm View spectra spectra

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10392557

Product Specific Information

The Rat anti-Mouse CD45 Monoclonal Antibody (mAb) recognizes all isoforms of the CD45 antigen. Applications reported for this antibody include flow cytometry, immunohistochemistry (zinc- and formalin-fixed, paraffin embedded), and immunohistochemistry (acetone-fixed, frozen). For this PE-Texas Red™ conjugate: excitation 488 nm/peak emission 616 (nm).

If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Cells should be analyzed within 18 hours of staining for best results. Avoid light exposure with fluorochrome-conjugated antibodies. Use dim light during handling, incubation with cells, and prior to analysis.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)
phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme
Process(es)
activation of MAPK activity negative regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration leukocyte cell-cell adhesion response to gamma radiation dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation T cell proliferation B cell proliferation positive regulation of T cell proliferation positive regulation of MAPK cascade cell cycle phase transition positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes bone marrow development stem cell development negative regulation of peptidyl-tyrosine phosphorylation T cell receptor signaling pathway B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
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