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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD49d (Integrin alpha 4) Antibodies

          Invitrogen

          CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™

          11 Published Figures
          15 References
          View all (55) CD49d (Integrin alpha 4) antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (11)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          CD49d (Integrin alpha 4) Antibody (16-0492-82) in Flow

          Mouse splenocytes were stained with 0.25 µg of Rat IgG2b kappa Isotype Control (Product # 14-4031-85) (blue histogram) or 0.25 µg of CD49d Monoclonal Antibody, Functional Grade (purple histogram) followed by IgG2b Monoclonal Antibody, PE (Product # 12-4815-82). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)
          CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)

          Product Details

          16-0492-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 10 publications 10 publications

          Neutralization (Neu)

          -
          View 2 publications 2 publications

          Functional Assay (Functional)

          Assay-Dependent
          View 2 publications 2 publications

          Control (Ctrl)

          Assay-Dependent
          -

          Inhibition Assays (IA)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Rat / IgG2b, kappa

          Recommended Isotype Control

          Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          R1-2

          Conjugate

          Functional Grade Functional Grade Functional Grade
          • APC 
          • Biotin 
          • FITC
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 702
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_468969

          Product Specific Information

          Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).

          Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis. R1-2 has also been reported in in vitro functional assays.

          Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha

          View more View less

          Gene Aliases: CD49D; Itga4; Itga4B

          View more View less

          UniProt ID: (Mouse) Q00651

          View more View less

          Entrez Gene ID: (Mouse) 16401

          View more View less

          Function(s)
          fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding integrin
          Process(es)
          blood vessel remodeling positive regulation of leukocyte migration cell-matrix adhesion involved in ameboidal cell migration cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heart development single organismal cell-cell adhesion cell migration heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading receptor clustering leukocyte tethering or rolling diapedesis face development mesenchyme development chorio-allantoic fusion visceral serous pericardium development T cell migration negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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