Invitrogen

CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™

CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)

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CD49d (Integrin alpha 4) Antibody (16-0492-82) in Flow

Mouse splenocytes were stained with 0.25 µg of Rat IgG2b kappa Isotype Control (Product # 14-4031-85) (blue histogram) or 0.25 µg of CD49d Monoclonal Antibody, Functional Grade (purple histogram) followed by IgG2b Monoclonal Antibody, PE (Product # 12-4815-82). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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Product Details

16-0492-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 10 publications 10 publications

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (FN)

Assay-Dependent

View 2 publications 2 publications

Control (Ctrl)

Assay-Dependent

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R1-2

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_468969

Product Specific Information

Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).

Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis. R1-2 has also been reported in in vitro functional assays.

Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry

Fri Apr 09 00:00:00 EDT 2021

VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models.

Neoplasia (New York, N.Y.)

Riegler J,Gill H,Ogasawara A,Hedehus M,Javinal V,Oeh J,Ferl GZ,Marik J,Williams S,Sampath D,Schartner J,Carano RAD

16-0492 was used in Flow cytometry/Cell sorting to test if non-invasive imaging of tumor perfusion and vascular inflammation may be able to explain differences in T-cell infiltration in pre-clinical tumor models, relevant for treatment outcomes.

Tue Oct 01 00:00:00 EDT 2019

A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.

Immunity

Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis.

Frontiers in immunology

Kobori T,Hamasaki S,Kitaura A,Yamazaki Y,Nishinaka T,Niwa A,Nakao S,Wake H,Mori S,Yoshino T,Nishibori M,Takahashi H

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry

Wed Mar 27 00:00:00 EDT 2019

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry

Tue Oct 30 00:00:00 EDT 2018

Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology.

PLoS pathogens

Pyle CJ,Uwadiae FI,Swieboda DP,Harker JA

16-0492-82 was used in Flow Cytometry to show that during respiratory virus infection the prototypic inflammatory cytokine IL-6 is a critical anti-inflammatory regulator of viral induced immunopathology in the respiratory tract through its induction of IL-27.

Fri Sep 01 00:00:00 EDT 2017

The impact of ranitidine on monocyte responses in the context of solid tumors.

Oncotarget

Vila-Leahey A,Rogers D,Marshall JS

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-0492 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 EDT 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-0492 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 EDT 2016

A Novel Model of Asymptomatic Plasmodium Parasitemia That Recapitulates Elements of the Human Immune Response to Chronic Infection.

PloS one

Fontana MF,Baccarella A,Craft JF,Boyle MJ,McIntyre TI,Wood MD,Thorn KS,Anidi C,Bayat A,Chung MR,Hamburger R,Kim CY,Pearman E,Pham J,Tang JJ,Boon L,Kamya MR,Dorsey G,Feeney ME,Kim CC

16-0492 was used in Flow cytometry/Cell sorting to investigate aspects of the human immune response to asymptomatic persistent Plasmodium infection using a novel animal model.

Wed Aug 02 00:00:00 EDT 2017

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 16-0492-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Blocking of α4 integrin does not protect from acute ischemic stroke in mice.

Stroke

Langhauser F,Kraft P,Göb E,Leinweber J,Schuhmann MK,Lorenz K,Gelderblom M,Bittner S,Meuth SG,Wiendl H,Magnus T,Kleinschnitz C

16-0492 was used in Functional assays to cast doubt on the effectiveness of anti-CD49d as a stroke treatment.

Sun Jun 01 00:00:00 EDT 2014

Cutting edge: CD8+ recent thymic emigrants exhibit increased responses to low-affinity ligands and improved access to peripheral sites of inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Berkley AM,Fink PJ

16-0492-82 was used in Flow Cytometry to investigate if the maturation state of peripheral T cells impacts the response to bacterial infection due to intrinsic differences in TCR signaling.

Wed Oct 01 00:00:00 EDT 2014

Suppression of leaky expression of adenovirus genes by insertion of microRNA-targeted sequences in the replication-incompetent adenovirus vector genome.

Molecular therapy. Methods & clinical development

Shimizu K,Sakurai F,Tomita K,Nagamoto Y,Nakamura S,Katayama K,Tachibana M,Kawabata K,Mizuguchi H

16-0492 was used in Immunocytochemistry to investigate how insertion of microRNA-targeted sequences into replication-incompetent adenovirus vector genomes can suppress leaky expression of adenoviral genes.

Wed May 27 00:00:00 EDT 2015

SWAP-70 controls formation of the splenic marginal zone through regulating T1B-cell differentiation.

European journal of immunology

Chopin M,Quemeneur L,Ripich T,Jessberger R

16-0492-82 was used in Neutralization to study the role of SWAP-70 in the differentiation, migration, and homing of marginal zone B cells and their precursors.

Wed Dec 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha

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Gene Aliases: CD49D; Itga4; Itga4B

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UniProt ID: (Mouse) Q00651

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Entrez Gene ID: (Mouse) 16401

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Molecular Function: integrin

Function(s)

blood vessel remodeling positive regulation of leukocyte migration cell-matrix adhesion involved in ameboidal cell migration cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heart development single organismal cell-cell adhesion cell migration heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading receptor clustering leukocyte tethering or rolling diapedesis face development mesenchyme development chorio-allantoic fusion visceral serous pericardium development T cell migration negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration

Process(es)

fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™

CD49d (Integrin alpha 4) Antibody (16-0492-82) in Flow

Product Details

16-0492-82

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (FN)

Control (Ctrl)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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