Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), PE-eFluor™ 610, eBioscience™

Product Image Gallery

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow) — BALB/c mouse splenocytes were stained with CD3e Monoclonal Antibody, APC (Product # 17-0031-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, PE-eFluor 610 (Product # 61-4321-82) (left) or 0.125 µg of CD62L Monoclonal Antibody, PE-eFluor 610 (right). Cells in the lymphocyte gate were used for analysis.

CD62L (L-Selectin) Antibody in Immunocytochemistry (ICC/IF) — Fig. 4 CXCL1 recruits peripheral naive CD4 + T cells and induces their differentiation into Tregs in situ by activating the NF-kappaB/FOXP3 pathway. a The distributions of CD34 + blood vessels (red), CD62L + naive T cells (yellow), and FOXP3 + Tregs (green) within the TME of mammary tumors were detected using the tissue immunofluorescence assay. Scale bar = 5 mum. b The expression levels of CXCR2 in naive CD4 + T cells sorted from the mammary tumors were detected by flow cytometry. c Flow cytometry assay indicated that 10-30 ng/ml CXCL1 treatment for 24 h significantly increased the chemotaxis efficacy of naive CD4 + T cells, whereas CXCL1/CXCR2 blockage by 2 muM SB225002 (CXCR2 inhibitor) partially abrogated that effect. d-e The expression levels of FOXP3 in 293 T cells ( d ) and naive CD4 + T cells ( e ) when treated as indicated for 24 h were detected by Western blot assay and immunofluorescence assay, respectively. Scale bar = 3 mum. f-g The mRNA expression level ( f ) and the promoter activity of FOXP3 gene in naive CD4 + T cells when treated as indicated for 24 h were detected by qPCR and double luciferase reporter gene assay, respectively. h-i Western blot assay ( h ) and cell immunofluorescence assay ( i ) indicated that CXCL1 treatment (10-30 ng/ml) for 24 h induced the expression and nuclear translocation of NF-kappab subunit p65, while CXCL1/CXCR2 blockage partially abrogated that effect. Scale bar = 3 mum. j CHIP-PCR assay validated that CXCL1 treatment (10-30 ng/

Product Details

61-0621-82

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.25 µg/test	View 21 publications 21 publications

Product Specifications
Species Reactivity	Mouse
Published species	Not Applicable
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	MEL-14
Conjugate	PE-eFluor™ 610 Alexa Fluor 561 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 615 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 eFluor 450 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 555 NovaFluor Blue 585 NovaFluor Blue 610-30S NovaFluor Blue 660-40S NovaFluor Red 710 NovaFluor Yellow 590 NovaFluor Yellow 660
Excitation/Emission Max	565/606 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2815302

Product Specific Information

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Theranostic near-infrared-IIb emitting nanoprobes for promoting immunogenic radiotherapy and abscopal effects against cancer metastasis.

Nature communications

Li H,Wang M,Huang B,Zhu SW,Zhou JJ,Chen DR,Cui R,Zhang M,Sun ZJ

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Thu Dec 09 00:00:00 EST 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis.

Nature communications

Wang G,Xie L,Li B,Sang W,Yan J,Li J,Tian H,Li W,Zhang Z,Tian Y,Dai Y

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Thu Sep 30 00:00:00 EDT 2021

cDC1 Dependent Accumulation of Memory T Cells Is Required for Chronic Autoimmune Inflammation in Murine Testis.

Frontiers in immunology

Jing Y,Cao M,Zhang B,Long X,Wang X

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Fri Sep 24 00:00:00 EDT 2021

Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.

Cell communication and signaling : CCS

Li J,Wang S,Wang N,Zheng Y,Yang B,Wang X,Zhang J,Pan B,Wang Z

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Immunocytochemistry

Mon Aug 30 00:00:00 EDT 2021

Trichinella spiralis Paramyosin Induces Colonic Regulatory T Cells to Mitigate Inflammatory Bowel Disease.

Frontiers in cell and developmental biology

Hao C,Wang W,Zhan B,Wang Z,Huang J,Sun X,Zhu X

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression.

iScience

AbuEid M,McAllister DM,McOlash L,Harwig MC,Cheng G,Drouillard D,Boyle KA,Hardy M,Zielonka J,Johnson BD,Hill RB,Kalyanaraman B,Dwinell MB

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Sirt6 Regulates the Development of Medullary Thymic Epithelial Cells and Contributes to the Establishment of Central Immune Tolerance.

Frontiers in cell and developmental biology

Zhang Q,Liang Z,Zhang J,Lei T,Dong X,Su H,Chen Y,Zhang Z,Tan L,Zhao Y

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Wed Apr 21 00:00:00 EDT 2021

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Fri Apr 09 00:00:00 EDT 2021

Evaluation of DNA-Launched Virus-Like Particle Vaccines in an Immune Competent Mouse Model of Chikungunya Virus Infection.

Vaccines

Rayner JO,Kim JH,Roberts RW,Wood RR,Fouty B,Solodushko V

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Fri Apr 02 00:00:00 EDT 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

CD8⁺ T cells in the central nervous system of mice with herpes simplex infection are highly activated and express high levels of CCR5 and CXCR3.

Journal of neurovirology

Lind L,Svensson A,Thörn K,Krzyzowska M,Eriksson K

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Mon Jun 03 00:00:00 EDT 2019

MTOR signaling is essential for the development of thymic epithelial cells and the induction of central immune tolerance.

Autophagy

Liang Z,Zhang L,Su H,Luan R,Na N,Sun L,Zhao Y,Zhang X,Zhang Q,Li J,Zhang L,Zhao Y

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Fri Mar 22 00:00:00 EDT 2019

ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.

Nature communications

Cao Y,Trillo-Tinoco J,Sierra RA,Anadon C,Dai W,Mohamed E,Cen L,Costich TL,Magliocco A,Marchion D,Klar R,Michel S,Jaschinski F,Reich RR,Mehrotra S,Cubillos-Ruiz JR,Munn DH,Conejo-Garcia JR,Rodriguez PC

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Wed Mar 20 00:00:00 EDT 2019

Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment.

Cell communication and signaling : CCS

Wiechert L,Németh J,Pusterla T,Bauer C,De Ponti A,Manthey S,Marhenke S,Vogel A,Klingmüller U,Hess J,Angel P

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 61-0621-82) in Flow Cytometry

Sat Dec 15 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Ly-22; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen

Gene Aliases: AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Ly22; Lyam-1; Lyam1; Sell

UniProt ID: (Mouse) P18337

Entrez Gene ID: (Mouse) 20343

Function(s)

cell adhesion response to ATP regulation of apoptotic process positive regulation of neutrophil chemotaxis

Process(es)

protease binding protein binding carbohydrate binding cell adhesion molecule binding glycolipid binding

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), PE-eFluor™ 610, eBioscience™

Product Details

61-0621-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!