|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
CD69 is a 55-70 kD glycoprotein that under reducing conditions dissociates in 3 components (gp 26,28,34), differentially glycosylated forms of a 21kD protein. CD69 is an early activation antigen also called Activation Inducer Molecule (AIM) that is very early, before antigens such as CD25 and CD71 are expressed strongly on T-cells after activation. CD69 is also expressed by activated B-lymphocytes.
Analyte Specific Reagent
The impact of Nucleofection® on the activation state of primary human CD4 T cells.
MHCD6904 was used in flow cytometry to investigate the effects of transfection on freshly isolated primary CD4 T cell morphology, intracellular calcium levels, cell surface activation markers, and transcriptional activity
|Zhang M,Ma Z,Selliah N,Weiss G,Genin A,Finkel TH,Cron RQ||Journal of immunological methods (408:123)||2014|
Activation of human NK cells by Plasmodium-infected red blood cells.
MHCD6904 was used in flow cytometry to describe a protocol to assess human NK cell activation following in vitro stimulation with malaria-infected red blood cells.
|Horowitz A,Riley EM||Methods in molecular biology (Clifton, N.J.) (923:447)||2012|
Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies.
MHCD6904 was used in flow cytometry to elucidate the role of A2ARs in the regulation of immune response.
|Koshiba M,Rosin DL,Hayashi N,Linden J,Sitkovsky MV||Molecular pharmacology (55:614)||1999|